cDNA clones of the human c-fes mRNA were isolated. Nucleotide analysis showed that c-fes mRNA contains a 2514 nucleotide open reading frame, which could encode for a 93 kDa protein, and both 5' and 3' v-fes nonhomologous sequences. Primer extension experiments confirmed that the longest isolated cDNAs are about the same length as the entire human c-fes mRNA. Sequence comparison between human c-fes cDNA and the corresponding genomic regions identified a 5' viral-non homologous exon (exon 1) located 491 bp upstream from the first v-fes homologous exon. The genomic region surrounding c-fes exon 1 contains a CpG island and acts as a promoter in vitro. Analysis of the 5' end of mouse c-fes cDNA suggested that the 5' human and mouse gene structure are similar.
Characterization of human and mouse c-fes cDNA clones and identification of the 5' end of the gene / M. Alcalay, F. Antolini, W. J. Van de Ven, L. Lanfrancone, F. Grignani, P. G. Pelicci. - In: ONCOGENE. - ISSN 0950-9232. - 5:3(1990 Mar), pp. 267-75-275.
|Titolo:||Characterization of human and mouse c-fes cDNA clones and identification of the 5' end of the gene|
|Parole Chiave:||Animals; Sequence Homology, Nucleic Acid; Exons; Humans; Protein-Tyrosine Kinases; Amino Acid Sequence; Mice; Proto-Oncogenes; Pregnancy; RNA, Messenger; Base Sequence; Proto-Oncogene Proteins c-fes; Proto-Oncogene Proteins; Placenta; Restriction Mapping; DNA; Molecular Sequence Data; Methylation; Cell Line; Female; Gene Library|
|Settore Scientifico Disciplinare:||Settore MED/04 - Patologia Generale|
|Data di pubblicazione:||mar-1990|
|Appare nelle tipologie:||01 - Articolo su periodico|