Flow cytometry with the specific monoclonal antibody (MoAb) L31 was used to analyse the expression of HLA class I heavy chains not bound with beta 2-microglobulin (beta 2m) by neuroblastoma (NB) cell lines IMR-32 and LA-N-1. The cells, which express barely detectable amounts of beta 2m-free (L31-positive molecules) and beta 2m-complexed HLA class I antigens (W6.32- and BBM.1-reactive molecules), expressed MHC class I molecules not bound to light chains upon differentiation with either retinoic acid or serum starvation. The expression was not accompanied by an increase of surface heterodimers. Conversely, recombinant interferon-gamma (rIFN-gamma) treatment led IMR-32 and LA-N-1 cells to almost exclusively express beta 2m-complexes HLA class I heavy chains. Surface beta 2m-free MHC class I molecules displayed a molecular mass of approximately 45 kDa and did not bind exogenously added beta 2m. No changes in the synthesis of either HLA class I and beta 2m mRNAs or of L31 proteins were observed in differentiated NB cells, thus suggesting that the surface exposure of unusual HLA class I antigens is regulated post-translationally. These findings indicate that, in addition to activated lymphocytes, the surface expression of beta 2m-free class I heavy chains is a feature of other cell types, such as NB cells.

Expression of beta 2m-free HLA class I heavy chains in neuroblastoma cell lines / A. Marozzi, R. Meneveri, G. Bunone, C. De Santis, L. Lopalco, A. Beretta, A. Agresti, A. G. Siccardi, G. Della Valle, E. Ginelli. - In: SCANDINAVIAN JOURNAL OF IMMUNOLOGY. - ISSN 0300-9475. - 37:6(1993 Jun), pp. 661-667. [10.1111/j.1365-3083.1993.tb01680.x]

Expression of beta 2m-free HLA class I heavy chains in neuroblastoma cell lines

A. Marozzi
Primo
;
E. Ginelli
Ultimo
1993

Abstract

Flow cytometry with the specific monoclonal antibody (MoAb) L31 was used to analyse the expression of HLA class I heavy chains not bound with beta 2-microglobulin (beta 2m) by neuroblastoma (NB) cell lines IMR-32 and LA-N-1. The cells, which express barely detectable amounts of beta 2m-free (L31-positive molecules) and beta 2m-complexed HLA class I antigens (W6.32- and BBM.1-reactive molecules), expressed MHC class I molecules not bound to light chains upon differentiation with either retinoic acid or serum starvation. The expression was not accompanied by an increase of surface heterodimers. Conversely, recombinant interferon-gamma (rIFN-gamma) treatment led IMR-32 and LA-N-1 cells to almost exclusively express beta 2m-complexes HLA class I heavy chains. Surface beta 2m-free MHC class I molecules displayed a molecular mass of approximately 45 kDa and did not bind exogenously added beta 2m. No changes in the synthesis of either HLA class I and beta 2m mRNAs or of L31 proteins were observed in differentiated NB cells, thus suggesting that the surface exposure of unusual HLA class I antigens is regulated post-translationally. These findings indicate that, in addition to activated lymphocytes, the surface expression of beta 2m-free class I heavy chains is a feature of other cell types, such as NB cells.
RNA, Messenger; Antigens, Surface; Base Sequence; Interferon-gamma; Tumor Cells, Cultured; Humans; Histocompatibility Antigens Class I; Molecular Sequence Data; Flow Cytometry; Immunoglobulin Heavy Chains; Neuroblastoma; beta 2-Microglobulin
Settore BIO/13 - Biologia Applicata
giu-1993
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/196378
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