Eps8 is a recently identified SH3-containing substrate for tyrosine kinase receptors. To understand the role of eps8 in receptor-mediated signaling, we cloned cDNAs encoding proteins that bind to its SH3 domain. One of these cDNAs predicts the synthesis of an 828 amino acid protein with homology to the N-terminal region of the tre oncogene. We designated this protein RN-tre for Related to the N-terminus of tre. RN-tre is ubiquitously expressed and maps to 10p13, a region known to be involved in translocations in various leukemias. In addition, a 10p13 monosomy syndrome, characterized by developmental alterations, has been reported. The regional homology between RN-tre and tre, which is limited to their N-terminal portion, prompted us to investigate the origin of the tre oncogene transcriptional unit. We were able to show that tre is the fusion product of a 5' genetic element, homologous to RN-tre and a 3' element, encoding a de-ubiquinating enzyme. Moreover, we identified, within the N-terminus of RN-tre and tre, a domain (named TrH, for Tre Homology), which is conserved within several proteins from yeast to mammals and has protein-binding properties in vitro.
|Titolo:||RN-tre identifies a family of tre-related proteins displaying a novel potential protein binding domain|
DI FIORE, PIER PAOLO (Ultimo)
|Parole Chiave:||Animals; Intracellular Signaling Peptides and Proteins; GTPase-Activating Proteins; Humans; Chromosomes, Human, Pair 10; Transcription, Genetic; Endopeptidases; Chromosome Mapping; src Homology Domains; DNA, Complementary; Adaptor Proteins, Signal Transducing; Molecular Sequence Data; Cytoskeletal Proteins; Sequence Homology, Amino Acid; Oncogene Proteins, Fusion; Ubiquitin Thiolesterase; 3T3 Cells; Oncogene Proteins; Amino Acid Sequence; Mice; Recombinant Fusion Proteins; Evolution, Molecular; Binding Sites; Base Sequence; Proto-Oncogene Proteins; Conserved Sequence; Proteins|
|Settore Scientifico Disciplinare:||Settore MED/04 - Patologia Generale|
|Data di pubblicazione:||20-giu-1996|
|Appare nelle tipologie:||01 - Articolo su periodico|