We developed a biocatalyst by cloning the styrene monooxygenase genes (styA and styB) from Pseudomonas fluorescens ST responsible for the oxidation of styrene to its corresponding epoxide. Recombinant Escherichia coli was able to oxidize different aryl vinyl and aryl ethenyl compounds to their corresponding optically pure epoxides. The results of bioconversion indicate the broad substrate preference of styrene monooxygenase and its potential for the production of several fine chemicals.

A new biocatalyst for production of optically pure aryl epoxides by styrene monooxygenase from Pseudomonas fluorescens ST / P. Di Gennaro, A. Colmegna, E.A. Galli, G. Sello, F. Pelizzoni, G. Bestetti. - In: APPLIED AND ENVIRONMENTAL MICROBIOLOGY. - ISSN 0099-2240. - 65:6(1999), pp. 2794-2797.

A new biocatalyst for production of optically pure aryl epoxides by styrene monooxygenase from Pseudomonas fluorescens ST

E.A. Galli;G. Sello;
1999

Abstract

We developed a biocatalyst by cloning the styrene monooxygenase genes (styA and styB) from Pseudomonas fluorescens ST responsible for the oxidation of styrene to its corresponding epoxide. Recombinant Escherichia coli was able to oxidize different aryl vinyl and aryl ethenyl compounds to their corresponding optically pure epoxides. The results of bioconversion indicate the broad substrate preference of styrene monooxygenase and its potential for the production of several fine chemicals.
Settore CHIM/06 - Chimica Organica
Settore BIO/11 - Biologia Molecolare
Settore CHIM/11 - Chimica e Biotecnologia delle Fermentazioni
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/196007
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