Several signal transducers bind through their SH2 domains to phosphotyrosine-containing motifs present in receptor tyrosine kinases (RTKs). However, the juxtamembrane regions of the epidermal growth factor receptor (EGFR) and of the related erbB-2 protein, while important in mitogenic signaling, lack demonstrable tyrosine phosphorylation sites, suggesting that other modalities of receptor-transducer interactions exist. A candidate for investigating this type of association is p97eps8, a recently described substrate for RTKs. p97eps8 is phosphorylated by several RTKs, associates with EGFR in vivo and, upon overexpression, enhances the transduction of EGFR-mediated mitogenic signals. Here we report that eps8 binds directly to the juxtamembrane region of EGFR through a domain that does not bear resemblance to SH2 domains and by a mechanism that does not require the presence of phosphotyrosine residues. Thus, the physical association between EGFR and eps8 represents a novel interaction between RTKs and their substrates.
|Titolo:||Direct binding of eps8 to the juxtamembrane domain of EGFR is phosphotyrosine- and SH2-independent|
DI FIORE, PIER PAOLO (Ultimo)
|Parole Chiave:||3T3 Cells; Animals; Phosphotyrosine; Receptor, Epidermal Growth Factor; Recombinant Proteins; Tyrosine; Amino Acid Sequence; Mice; Protein Binding; Structure-Activity Relationship; Receptor Protein-Tyrosine Kinases; Adaptor Proteins, Signal Transducing; Molecular Sequence Data; Cytoskeletal Proteins; Proteins; Signal Transduction|
|Settore Scientifico Disciplinare:||Settore MED/04 - Patologia Generale|
|Data di pubblicazione:||16-feb-1995|
|Appare nelle tipologie:||01 - Articolo su periodico|