Introduction of the normal erbB-2 gene into immortalized human mammary epithelial cells (184B5) by transfection conferred a growth advantage to these cells both in vitro and in vivo. The 184B5 cells overexpressing erbB-2 formed colonies in semi-solid medium, frequently induced transient nodules in athymic mice and produced progressive tumors in vivo at a low frequency. Those tumors which did arise from erbB-2-transfected cells displayed substantially higher levels of normal gp185erb-2 protein when compared to the original transfectants, consistent with their selection for increased erbB-2 expression. Introduction of genes encoding genetically altered erbB-2 molecules into 184B5 cells increased their colony-forming efficiency and converted the cells to a tumorigenic phenotype at a high frequency. When the biological and biochemical properties of human mammary carcinoma cell lines known to overexpress erbB-2 were compared to the transfected 184B5 lines, they behaved most like those overexpressing the normal erbB-2 protein. Results indicate that overexpression of normal erbB-2 may directly contribute to the transformation of human mammary epithelium if sufficient levels of erbB-2 protein are expressed or if the erbB-2 gene is genetically altered.
|Titolo:||Oncogenic potential of erbB-2 in human mammary epithelial cells|
DI FIORE, PIER PAOLO (Penultimo)
|Parole Chiave:||Animals; Phosphotyrosine; Receptor, erbB-2; Humans; Breast; Gene Expression; Tyrosine; Protein-Tyrosine Kinases; Breast Neoplasms; Mice; Mice, Nude; Proto-Oncogenes; Neoplasm Transplantation; Proto-Oncogene Proteins; Phosphorylation; Transfection; DNA; Epithelium; Cell Line; Cell Transformation, Neoplastic|
|Settore Scientifico Disciplinare:||Settore MED/04 - Patologia Generale|
|Data di pubblicazione:||lug-1991|
|Appare nelle tipologie:||01 - Articolo su periodico|