The epidermal growth factor (EGF) receptor (EGFR) and the erbB-2 gene product, gp185erbB-2, exhibit distinct abilities to stimulate mitogenesis in different target cells. By using chimeric molecules between these two receptors, we have previously shown that their intracellular juxtamembrane regions are responsible for this specificity. Here we describe a genetically engineered EGFR mutant containing a threonine for arginine substitution at position 662 in the EGFR juxtamembrane domain, corresponding to threonine 694 in gp185erbB-2. This mutant, designated EGFRThr662, displayed affinity for EGF binding and catalytic properties that were indistinguishable from those of the wild type EGFR. However, EGFRThr662 behaved much as gp185erbB-2 in a number of bioassays which readily distinguish between the mitogenic effects of EGFR and gp185erbB-2. Moreover, significant differences were detected in the pattern of intracellular proteins phosphorylated on tyrosine in vivo by EGFR and EGFRThr662 in response to EGF. Thus, small differences in the primary sequence of two closely related receptors have dramatic effects on their ability to couple with mitogenic pathways.
|Titolo:||A single amino acid substitution is sufficient to modify the mitogenic properties of the epidermal growth factor receptor to resemble that of gp185erbB-2|
DI FIORE, PIER PAOLO (Primo)
|Parole Chiave:||Codon; Animals; Epidermal Growth Factor; Mutagenesis, Site-Directed; Phosphorylation; Genetic Vectors; Molecular Sequence Data; Mitogens; Repetitive Sequences, Nucleic Acid; DNA Replication; Signal Transduction; 3T3 Cells; Oligodeoxyribonucleotides; Arginine; Receptor, Epidermal Growth Factor; Receptor, erbB-2; Protein-Tyrosine Kinases; Mice; Amino Acid Sequence; Proto-Oncogenes; Base Sequence; Proto-Oncogene Proteins; Transfection; Kinetics; Threonine; Thymidine|
|Settore Scientifico Disciplinare:||Settore MED/04 - Patologia Generale|
|Data di pubblicazione:||nov-1992|
|Appare nelle tipologie:||01 - Articolo su periodico|