Six basic acrylamido buffers, for isoelectric focusing in immobilized pH gradients, are described: a strong titrant (pK greater than 12), QAE-acrylamide, and five weaker bases, namely: 2-morpholinoethyl acrylamide (pK 6.2), 3-morpholinopropyl acrylamide (pK 7.0), N,N-dimethyl aminoethyl acrylamide (pK 8.5), N,N-dimethyl aminopropyl acrylamide (pK 9.3) and N,N-diethyl aminopropyl acrylamide (pK 10.3). Their synthesis and general properties are described.
Synthesis of buffers for generating immobilized pH gradients. II: Basic acrylamido buffers / M. Chiari, E. Casale, E. Santaniello, P.G. Righetti. - In: APPLIED AND THEORETICAL ELECTROPHORESIS. - ISSN 0954-6642. - 1:2(1989), pp. 103-107.
Synthesis of buffers for generating immobilized pH gradients. II: Basic acrylamido buffers.
E. SantanielloPenultimo
;
1989
Abstract
Six basic acrylamido buffers, for isoelectric focusing in immobilized pH gradients, are described: a strong titrant (pK greater than 12), QAE-acrylamide, and five weaker bases, namely: 2-morpholinoethyl acrylamide (pK 6.2), 3-morpholinopropyl acrylamide (pK 7.0), N,N-dimethyl aminoethyl acrylamide (pK 8.5), N,N-dimethyl aminopropyl acrylamide (pK 9.3) and N,N-diethyl aminopropyl acrylamide (pK 10.3). Their synthesis and general properties are described.
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