Five acidic acrylamido buffers for isoelectric focusing in immobilized pH gradients, are described: a strong titrant (pK 1.0) 2-acrylamido-2-methyl propane sulphonic acid and four weaker acids, namely 2-acrylamido glycolic acid (pK 3.1), N-acryloil glycine (pK 3.6), 3-acrylamido propanoic acid (pK 4.4) and 4-acrylamido butyric acid (pK 4.6). Their synthesis and general properties are described.
Synthesis of buffers for generating immobilized pH gradients. I: Acidic acrylamido buffers / M. Chiari, E. Casale, E. Santaniello, P.G. Righetti. - In: APPLIED AND THEORETICAL ELECTROPHORESIS. - ISSN 0954-6642. - 1:2(1989), pp. 99-102.
Synthesis of buffers for generating immobilized pH gradients. I: Acidic acrylamido buffers.
E. SantanielloPenultimo
;
1989
Abstract
Five acidic acrylamido buffers for isoelectric focusing in immobilized pH gradients, are described: a strong titrant (pK 1.0) 2-acrylamido-2-methyl propane sulphonic acid and four weaker acids, namely 2-acrylamido glycolic acid (pK 3.1), N-acryloil glycine (pK 3.6), 3-acrylamido propanoic acid (pK 4.4) and 4-acrylamido butyric acid (pK 4.6). Their synthesis and general properties are described.
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