A wide variety of human tumors contain an amplified or overexpressed erbB-2 gene, which encodes a growth factor receptor-like protein. When erbB-2 complementary DNA was expressed in NIH/3T3 cells under the control of the SV40 promoter, the gene lacked transforming activity despite expression of detectable levels of the erbB-2 protein. A further five- to tenfold increase in its expression under influence of the long terminal repeat of Moloney murine leukemia virus was associated with activation of erbB-2 as a potent oncogene. The high levels of the erbB-2 product associated with malignant transformation of NIH/3T3 cells were observed in human mammary tumor cells that overexpressed this gene. These findings demonstrate a new mechanism for acquisition of oncogenic properties by genes encoding growth factor receptor-like proteins and provide a functional basis for the role of their overexpression in the development of human malignancies.
|Titolo:||erbB-2 is a potent oncogene when overexpressed in NIH/3T3 cells|
DI FIORE, PIER PAOLO (Primo)
|Parole Chiave:||Animals; Moloney murine leukemia virus; Receptor, erbB-2; Receptor, Epidermal Growth Factor; Humans; Breast Neoplasms; Recombinant Fusion Proteins; Mice; Receptors, Cell Surface; Fibroblasts; Rats; Promoter Regions, Genetic; Proto-Oncogene Proteins; Simian virus 40; DNA; Gene Expression Regulation; Genes, Viral; Tumor Stem Cell Assay; Cell Transformation, Neoplastic; Cell Line|
|Settore Scientifico Disciplinare:||Settore MED/04 - Patologia Generale|
|Data di pubblicazione:||10-lug-1987|
|Digital Object Identifier (DOI):||10.1126/science.2885917|
|Appare nelle tipologie:||01 - Articolo su periodico|