OBJECTIVE: Tp assess the frequency of the presence and th with laboratory findings and clinical conditions of CCIC patients with CMV infection. METHODS: CMV infection routine diagnosis (viral isolation and determination of pp65 antigenemia and p72 viremia) was performed on 1737 blood samples (buffy-coat cells) from 450 HIV infected patients. Ficoll separation of mononuclear cells and PCR amplification of a IE1 gene region of CMV was performed on selected cases. RESULTS: CCIC were found in 30 samples from 21 patients: Eleven specimens were positive in the course of anti-CMV therapy. All positive samples were also pp65, p72 and isolation positive. Ficoll separation allowed a greater number of CCIC to be found. PCR was positive in all but one of the CCIC positive samples. DISCUSSION: CCIC were not frequently found; their systematic search in the mononuclear cell fraction could contribute to the understanding of their role.
CIRCULATING CMV-INFECTED ENDOTHELIAL CELLS (CCIC) IN AIDS PAITIENTS WITH CMV RELATED SYMPTOMS / M. Barbi, S. Binda, V. Primache, C. Luraschi, S. Montano, L. Testa. ((Intervento presentato al 4°. convegno European Conference on clinical aspects and treatment of HIV infection tenutosi a Milano nel 1994.
CIRCULATING CMV-INFECTED ENDOTHELIAL CELLS (CCIC) IN AIDS PAITIENTS WITH CMV RELATED SYMPTOMS
M. BarbiPrimo
;S. BindaSecondo
;V. Primache;
1994
Abstract
OBJECTIVE: Tp assess the frequency of the presence and th with laboratory findings and clinical conditions of CCIC patients with CMV infection. METHODS: CMV infection routine diagnosis (viral isolation and determination of pp65 antigenemia and p72 viremia) was performed on 1737 blood samples (buffy-coat cells) from 450 HIV infected patients. Ficoll separation of mononuclear cells and PCR amplification of a IE1 gene region of CMV was performed on selected cases. RESULTS: CCIC were found in 30 samples from 21 patients: Eleven specimens were positive in the course of anti-CMV therapy. All positive samples were also pp65, p72 and isolation positive. Ficoll separation allowed a greater number of CCIC to be found. PCR was positive in all but one of the CCIC positive samples. DISCUSSION: CCIC were not frequently found; their systematic search in the mononuclear cell fraction could contribute to the understanding of their role.Pubblicazioni consigliate
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