Deletions of the long arm of chromosome 6 (6q-) are frequently found in hematopoietic neoplasms, including acute lymphoblastic leukemias, non-Hodgkin lymphomas and (less frequently) myeloid leukemias. The c-myb proto-oncogene has been mapped to region 6q21-24, which suggests that it could be involved in the 6q- aberrations. By means of in situ chromosomal hybridization on cells from six hematopoietic malignancies, it was demonstrated that the c-myb locus is not deleted, but is retained on band q22, which is consistently bordered by the chromosomal breakpoints in both interstitial and terminal 6q- deletions. The deletion breakpoints were located at some distance from the myb locus since no rearrangement of c-myb sequences was found. In one case, however, amplification of the entire c-myb locus was detectable. Furthermore, in all cases tested that carry 6q- deletions, myb messenger RNA levels were significantly higher than in normal cells or in malignant cells matched for lineage and stage of differentiation but lacking the 6q- marker. These results indicate that 6q- deletions are accompanied by structural and functional alterations of the c-myb locus and that these alterations may be involved in the pathogenesis of leukemias and lymphomas.
|Titolo:||Relationship between the c-myb locus and the 6q-chromosomal aberration in leukemias and lymphomas|
PELICCI, PIER GIUSEPPE (Secondo)
|Parole Chiave:||RNA, Messenger; Leukemia; Leukemia, Lymphoid; Chromosome Deletion; Humans; DNA; Nucleic Acid Hybridization; Leukemia, Myeloid; Lymphoma, Non-Hodgkin; Chromosomes, Human, Pair 6; Gene Amplification|
|Settore Scientifico Disciplinare:||Settore MED/04 - Patologia Generale|
|Data di pubblicazione:||27-feb-1987|
|Appare nelle tipologie:||01 - Articolo su periodico|