The possible occurrence of sialyltransferase activity in the plasma membranes surrounding nerve endings (synaptosomal membranes) was studied, using calf brain cortex. The synaptosomal membranes were prepared by an improved procedure which provided: (a) a 'nerve ending fraction' consisting of at least 85% well-preserved nerve endings and containing only small quantities of membranes of intracellular origin; (b) a 'synaptosomal membrane fraction' carrying high amounts of authentic plasma membrane markers (Na+-K+ ATPase, 5'-nucleotidase, sialidase, gangliosides) with values of specific activity four to fivefold higher than those in the 'nerve ending fraction' and very small amounts of cerebroside sulphotransferase, marker of the Golgi apparatus, and of other markers of intracellular membranes (rotenone-insensitive NADH and NADPH: cytochrome c reductases), the specific activities of which were, respectively, 0.5- and 0.7-fold that in the 'nerve ending fraction'. Thus the preparation of synaptosomal membranes used had the characteristics of plasma membranes and carried a negligible contamination of membranes of intracellular origin. The distribution of sialyltransferase activity in the main brain subcellular fractions (microsomes; P2 fraction; nerve ending fraction; mitochondria) resembled most closely that of thiamine pyrophosphatase, the enzyme known to be linked to the Golgi apparatus and the plasma membranes and of acetylcholine esterase, the enzyme known to be linked to either intracellular or plasma membranes. The enrichment of sialyltransferase activity in the 'synaptosomal membrane fraction', referred to the 'nerve ending fraction', was practically the same as that exhibited by authentic plasma membrane markers. All this is consistent with the hypothesis that in calf brain cortex sialyltransferase has two different subcellular locations: one at the level of intracellular structures, most likely the Golgi apparatus (as described by other authors), the other in the synaptosomal plasma membranes. The basic properties (pH optimum, V/S, V/t and V/protein relationships) and detergent requirements of the synaptosomal membrane-bound sialyltransferase were established. The highest enzyme activities were recorded on exogenous acceptors, lactosylceramide and DS-fetuin. The K values for CMP-NeuNAc were different using lactosylceramide and DS-fetuin as acceptor substrates (0.57 and 0.135 mM, respectively); the thermal stability of the enzyme acting on glycolipid acceptor was higher than that on the glycoprotein acceptor; the effect of detergents was different when using glycoprotein from glycolipid acceptors; no competition was observed between lactosylceramide and DS-fetuin. Thus the synaptosomal membranes carry at least two different sialyltransferase activities; one acting on lactosylceramide (and glycolipid acceptors), the other working on DS-fetuin (and glycoprotein acceptors). Ganglioside GM3 was recognized as the product of synaptosomal membrane-bound sialyltransferase activity working on lactosylceramide as acceptor substrate.
|Titolo:||occurrence of sialyltransferase activity in the synaptosomal membranes prepared from calf brain|
FIORILLI, AMELIA (Secondo)
|Parole Chiave:||synaptosomal membranes; sialyltransferase|
|Settore Scientifico Disciplinare:||Settore BIO/10 - Biochimica|
|Data di pubblicazione:||gen-1980|
|Digital Object Identifier (DOI):||10.1111/j.1471-4159.1980.tb06263.x|
|Appare nelle tipologie:||01 - Articolo su periodico|