Subcellular liver fractions from rats receiving a subcutaneous injection of turpentine, which causes a local inflammation, show an increased synthesis of Prostaglandin E2 and Prostaglandin F2 alpha which reaches a peak 90 minutes and 3 hours after treatment, respectively. Stimulation of phospholipase A2 activity of liver cell preparations seems to be responsible for the supply of arachidonic acid necessary to feed PG synthesis: this stimulation is accompanied by unchanged levels of diacylglycerol lipase, diacylglycerol kinase and protein kinase C activities and by an unchanged content of diacylglycerol in the liver tissue. This picture does not favour the hypothesis of an involvement of phospholipase C in the early stages after turpentine treatment. Determinations of GTP-ase activity in plasma membrane-rich liver preparations give ambiguous results, which do not allow any conclusion on the possible role of G-proteins in phospholipase A2 activation.
|Titolo:||Rat liver eicosanoid synthesis during turpentine-induced inflammation|
BENDINELLI, PAOLA (Penultimo)
|Parole Chiave:||Animals; Microsomes, Liver; Turpentine; Subcellular Fractions; Phospholipases A2; Hepatitis, Animal; Diglycerides; Dinoprost; Guanosine Triphosphate; Rats; Rats, Inbred Strains; Dinoprostone; Protein Kinase C; Male; Phospholipases A|
|Settore Scientifico Disciplinare:||Settore MED/04 - Patologia Generale|
|Data di pubblicazione:||1991|
|Appare nelle tipologie:||01 - Articolo su periodico|