We identified two types of gamma/delta T-cell clones: (1) BB3+ clones, which express the disulphide-linked form of the gamma/delta heterodimer (type 1 receptor) and carry rearrangements of the C gamma 1 gene; and (2) delta TCS1+ clones, which express the nondisulphide-linked form of the gamma/delta heterodimer (type 2 receptor) and carry rearrangements of the C gamma 2 gene. To determine the joining (J) and variable (V) region usage of the two receptor types, T gamma and T delta gene rearrangements and expression were analyzed in each gamma/delta T-cell clone. The results showed that the J and V regions used by the two receptor types are distinct: clones that express the type 1 receptor carry potentially functional rearrangements in J gamma P-V gamma 9 for gamma and in J delta 1-V delta 2 for delta; and clones that express the type 2 receptor had potentially functional rearrangements in J gamma 2-V gamma 2/V gamma 4 for gamma and in J delta 1-V delta 1 for delta. Therefore, two clearly different gamma/delta receptors (V gamma 9-J gamma P-C gamma 1/V delta 2-J delta 1-C delta and V gamma 2/V gamma 4-J gamma 2-C gamma 2/V delta 1-J delta 1-C delta) predominate in peripheral blood.
|Titolo:||The C gamma 1-encoded disulphide-linked and the C gamma 2-encoded nondisulphide-linked forms of the gamma/delta heterodimer use different gamma and delta variable regions|
PELICCI, PIER GIUSEPPE (Ultimo)
|Parole Chiave:||Clone Cells; Deoxyribonuclease BamHI; Deoxyribonuclease EcoRI; Exons; Humans; Nucleic Acid Hybridization; Disulfides; Antibodies, Monoclonal; Macromolecular Substances; DNA Probes; Bacterial Proteins; Gene Rearrangement, T-Lymphocyte; Adult; Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor; DNA; Receptors, Antigen, T-Cell; Immunosorbent Techniques; Flow Cytometry; Deoxyribonucleases, Type II Site-Specific|
|Settore Scientifico Disciplinare:||Settore MED/04 - Patologia Generale|
|Data di pubblicazione:||15-lug-1990|
|Appare nelle tipologie:||01 - Articolo su periodico|