On the basis of sequence similarity in the repeated zinc finger domain, we have identified and characterized two human cDNA clones (ZNF7 and ZNF8), both encoding proteins containing potential finger-like nucleic acid binding motifs. Northern blot analysis indicates that both genes are expressed as multiple transcripts and they are ubiquitously present in many human cell lines of different embryological derivation. Moreover, their expression is modulated during in vitro induced terminal differentiation of human myeloid cell line HL-60. By in situ hybridization experiments, we have localized the ZNF7 gene to chromosome 8 (region q24) and the ZNF8 gene to the terminal band of the long arm of chromosome 20 (20q13).
|Titolo:||cDNA isolation, expression analysis, and chromosomal localization of two human zinc finger genes|
PELICCI, PIER GIUSEPPE (Ultimo)
|Parole Chiave:||Blotting, Northern; Sequence Homology, Nucleic Acid; Humans; DNA-Binding Proteins; Metalloproteins; Kruppel-Like Transcription Factors; Gene Expression; Cell Differentiation; Transcription, Genetic; Chromosomes, Human, Pair 8; Amino Acid Sequence; Chromosome Mapping; Cloning, Molecular; Base Sequence; Genes; Chromosomes, Human, Pair 20; Zinc; Molecular Sequence Data; DNA; Female; Cell Line|
|Settore Scientifico Disciplinare:||Settore MED/04 - Patologia Generale|
|Data di pubblicazione:||feb-1990|
|Digital Object Identifier (DOI):||10.1016/0888-7543(90)90574-E|
|Appare nelle tipologie:||01 - Articolo su periodico|