Alterations of chromosomes 7 and 11 have been involved in the progression of atherosclerosis. Twenty-three carotid endarterectomy specimens were studied for the presence of alterations in chromosomes 7 and 11, and fibroblastic growth factor-3 (FGF-3) gene amplification. Besides classic histological stainings, immunophenotyping of cellular and vascular components and fluorescence in situ hybridization (FISH) were performed. At the caps, unstable plaques (n=18) showed inflammatory infiltration of macrophages, smooth muscle cells, and T-lymphocytes. Specifically in these regions, the FISH showed varying percentages of trisomy (15/18) and tetrasomy (8/15) of chromosome 7. In four cases polisomy 7 was noted in some nuclei. Monosomy of chromosome 11 and gene amplification of FGF-3 gene was observed. The FISH of the five stable plaques and normal arterial walls showed no chromosome alterations; furthermore, chromosome 3, which is not involved in atherosclerotic progression, presented a normal ploidy of smooth muscle cells in stable and unstable plaques and normal arterial walls. In conclusion, chromosome 7 and 11 alterations and FGF-3 gene amplification are components of unstable plaques, and might contribute to the evolution of stable plaques into complicated plaques.
Chromosomal alterations in atherosclerotic plaques / L. Matturri, A. Cazzullo, P. Turconi, A.M. Lavezzi, P.L. Vandone, L. Gabrielli, G. Fernández Alonso, D. Grana, J. Milei. - In: ATHEROSCLEROSIS. - ISSN 0021-9150. - 154:3(2001 Mar), pp. 755-761.
|Titolo:||Chromosomal alterations in atherosclerotic plaques|
|Parole Chiave:||Atherosclerosis; Carotid artery; Chromosomal alterations; Chromosome 11; Chromosome 7; Fibroblastic growth factor-3, (FGF-3); Genetic alterations|
|Settore Scientifico Disciplinare:||Settore MED/08 - Anatomia Patologica|
Settore BIO/18 - Genetica
|Data di pubblicazione:||mar-2001|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1016/S0021-9150(00)00488-3|
|Appare nelle tipologie:||01 - Articolo su periodico|