RAR and AML1 transcription factors are found in leukemias as fusion proteins with PML and ETO, respectively. Association of PML-RAR and AML1-ETO with the nuclear corepressor (N-CoR)/histone deacetylase (HDAC) complex is required to block hematopoietic differentiation. We show that PML-RAR and AML1-ETO exist in vivo within high molecular weight (HMW) nuclear complexes, reflecting their oligomeric state. Oligomerization requires PML or ETO coiled-coil regions and is responsible for abnormal recruitment of N-CoR, transcriptional repression, and impaired differentiation of primary hematopoietic precursors. Fusion of RAR to a heterologous oligomerization domain recapitulated the properties of PML-RAR, indicating that oligomerization per se is sufficient to achieve transforming potential. These results show that oligomerization of a transcription factor, imposing an altered interaction with transcriptional coregulators, represents a novel mechanism of oncogenic activation.

Oligomerization of RAR and AML1 transcription factors as a novel mechanism of oncogenic activation / S. Minucci, M. Maccarana, M. Cioce, P. De Luca, V. Gelmetti, S. Segalla, L. Di Croce, S. Giavara, C. Matteucci, A. Gobbi, A. Bianchini, E. Colombo, I. Schiavoni, G. Badaracco, X. Hu, M. A. Lazar, N. Landsberger, C. Nervi, P. G. Pelicci. - In: MOLECULAR CELL. - ISSN 1097-2765. - 5:5(2000 May), pp. 811-820.

Oligomerization of RAR and AML1 transcription factors as a novel mechanism of oncogenic activation

S. Minucci;E. Colombo;N. Landsberger;P. G. Pelicci
2000

Abstract

RAR and AML1 transcription factors are found in leukemias as fusion proteins with PML and ETO, respectively. Association of PML-RAR and AML1-ETO with the nuclear corepressor (N-CoR)/histone deacetylase (HDAC) complex is required to block hematopoietic differentiation. We show that PML-RAR and AML1-ETO exist in vivo within high molecular weight (HMW) nuclear complexes, reflecting their oligomeric state. Oligomerization requires PML or ETO coiled-coil regions and is responsible for abnormal recruitment of N-CoR, transcriptional repression, and impaired differentiation of primary hematopoietic precursors. Fusion of RAR to a heterologous oligomerization domain recapitulated the properties of PML-RAR, indicating that oligomerization per se is sufficient to achieve transforming potential. These results show that oligomerization of a transcription factor, imposing an altered interaction with transcriptional coregulators, represents a novel mechanism of oncogenic activation.
Histone Deacetylases ; Humans ; Transcription, Genetic ; Leukemia, Myeloid ; Protein Binding ; Nuclear Receptor Co-Repressor 1 ; Protein Structure, Quaternary ; Leukemia ; Peptide Fragments ; Neoplasm Proteins ; Transcription Factors ; Nuclear Proteins ; Repressor Proteins ; Leukemia, Promyelocytic, Acute ; Tretinoin ; Core Binding Factor Alpha 2 Subunit ; Response Elements ; Cell Transformation, Neoplastic ; Oncogene Proteins, Fusion
Settore MED/04 - Patologia Generale
mag-2000
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/195095
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