Vascular endothelial growth factor (VEGF) and metalloproteinase (MMP) 2 and 9 are useful biomarkers in human lymphoma. During cancerogenesis, transforming growth factor beta (TGF-β) stimulates VEGF and MMPs production. VEGF and TGF-β plasma levels were tested by ELISA, MMP-2 and MMP-9 by gelatine zymography in 37 dogs with lymphoma, 13 of which were also monitored during chemotherapy. Ten healthy dogs served as control. Lymphoma dogs showed higher act-MMP-9 (P<0.01) and VEGF (P<0.05), and lower TGF-β than controls, and a positive correlation between act-MMP-9 and VEGF (P<0.001). Act-MMP-9 and VEGF were significantly higher in T-cell lymphomas, and in stage V compared with stages III-IV disease, regardless of immunophenotype. VEGF was higher in high-grade compared with low-grade T-cell lymphomas. No correlation was found between cytokines levels at presentation and outcome. During chemotherapy, act-MMP-9 and VEGF decreased in B-cell lymphomas (P<0.01), suggesting a possible predictive role in this group of dogs.

VEGF and MMP-9 : biomarkers for canine lymphoma / L. Aresu, A. Aricò, S. Comazzi, M.E. Gelain, F. Riondato, M. Mortarino, E. Morello, D. Stefanello, M. Castagnaro. - In: VETERINARY AND COMPARATIVE ONCOLOGY. - ISSN 1476-5810. - 12:1(2014), pp. 29-36. [10.1111/j.1476-5829.2012.00328.x]

VEGF and MMP-9 : biomarkers for canine lymphoma

S. Comazzi;M. Mortarino;D. Stefanello
Penultimo
;
2014

Abstract

Vascular endothelial growth factor (VEGF) and metalloproteinase (MMP) 2 and 9 are useful biomarkers in human lymphoma. During cancerogenesis, transforming growth factor beta (TGF-β) stimulates VEGF and MMPs production. VEGF and TGF-β plasma levels were tested by ELISA, MMP-2 and MMP-9 by gelatine zymography in 37 dogs with lymphoma, 13 of which were also monitored during chemotherapy. Ten healthy dogs served as control. Lymphoma dogs showed higher act-MMP-9 (P<0.01) and VEGF (P<0.05), and lower TGF-β than controls, and a positive correlation between act-MMP-9 and VEGF (P<0.001). Act-MMP-9 and VEGF were significantly higher in T-cell lymphomas, and in stage V compared with stages III-IV disease, regardless of immunophenotype. VEGF was higher in high-grade compared with low-grade T-cell lymphomas. No correlation was found between cytokines levels at presentation and outcome. During chemotherapy, act-MMP-9 and VEGF decreased in B-cell lymphomas (P<0.01), suggesting a possible predictive role in this group of dogs.
Dog Lymphoma; canine lymphoma; MMP-9; prognosis; VEGF
Settore VET/03 - Patologia Generale e Anatomia Patologica Veterinaria
Settore VET/08 - Clinica Medica Veterinaria
Settore VET/09 - Clinica Chirurgica Veterinaria
Settore VET/06 - Parassitologia e Malattie Parassitarie degli Animali
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/195007
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