The possible relationship between metabolism and biological effects of sphingosine was investigated in Neuro2a cells. [C3-3H]-sphingosine, administered at different doses (80 pmol-80 nmol/mg cell protein). Amounts up to hundredfold were rapidly taken up and metabolized, the intracellular content of sphingosine being processed within 2h. At low doses, [3H]-sphingosine represented a minor portion of the cellular radiolabel, and N-acylated metabolites, particularly ceramide, prevailed over degradation products. Neuro2a cell differentiation took place in conjunction with ceramide increase. At increasing exogenous sphingosinc/cell ratio, the acylation process became saturated while sphingosine degradation increased proportionally. From this point on [3H]-sphingosinc accumulated and cell toxicity occurred. In conclusion, in Neuro2a cells the biological effects exerted by exogenous sphingosine are strictly connected to the exogenous sphingosine/cell ratio and to the capacity of the cell to metabolize sphingosine.
Metabolic Fate of Exogenous Sphingosine in Neuroblastoma Neuro2A Cells: Dose-dependence and Biological Effectsa / L. RIBONI, R. BASSI, A. CAMINITI, A. PRINETTI, P. VIANI, G. TETTAMANTI. - In: ANNALS OF THE NEW YORK ACADEMY OF SCIENCES. - ISSN 0077-8923. - 845:1 SPHINGOLIPIDS(1998), pp. 46-56.
|Titolo:||Metabolic Fate of Exogenous Sphingosine in Neuroblastoma Neuro2A Cells: Dose-dependence and Biological Effectsa|
RIBONI, LAURA PIERA CARLA GIULIA (Primo)
BASSI, ROSARIA (Secondo)
VIANI, PAOLA (Penultimo)
TETTAMANTI, GUIDO (Ultimo)
|Settore Scientifico Disciplinare:||Settore BIO/10 - Biochimica|
|Data di pubblicazione:||1998|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1111/j.1749-6632.1998.tb09661.x|
|Appare nelle tipologie:||01 - Articolo su periodico|