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EnglishWe have examined the type of inhibition exerted by an activated preparation of the Bacillus thuringiensis delta-endotoxin CrylAa on K+-dependent leucine transport into midgut brush border membrane vesicles or epithelial cells of the isolated midgut from Bombyx mori to study its possible interaction with the amino acid symporter. K+ permeability and the cation-dependent amino acid translocation into brush border membrane vesicles were evaluated by monitoring the fluorescence of the voltage-sensitive cyanine dye 3,3'-dipropylthiadicarbocyanine iodide. The symporter ability to accept Na+ instead of K+ was exploited and the dissipation of an imposed inside-negative potential (K+ gradient in>out and valinomycin) was registered in the presence of a Na+ gradient (out>in) and of the amino acid. The fluorescence quenching dissipated more rapidly when the amino acid was present. Preincubation of brush border membrane vesicles with CrylAa caused a significant decrease of the amino acid-dependent recovery of fluorescence, whereas K+ permeability was sparely affected. In the isolated midgut, CrylAa inhibits leucine uptake as well as the transepithelial electrical potential difference. The strong inhibition exerted by the delta-endotoxin was observed also in the absence of potassium and the transepithelial electrical potential difference. The results obtained strongly suggest a direct interaction of CrylAa delta-endotoxin with the K+/amino acid symporter.
Bacillus thuringiensis CrylAa δ-endotoxin affects the K+/amino acid symport in Bombyx mori larval midgut / M.G. Leonardi, P. Parenti, M. Casartelli, B. Giordana. - In: THE JOURNAL OF MEMBRANE BIOLOGY. - ISSN 0022-2631. - 159:3(1997), pp. 209-217.
| Titolo: | Bacillus thuringiensis CrylAa δ-endotoxin affects the K+/amino acid symport in Bombyx mori larval midgut |
| Autori: | |
| Parole Chiave: | CrylAa delta-endotoxin ; Bacillus thuringiensis ; Bombyx mori larval midgut ; K+/amino acid symporters ; Brush border membrane vesicles ; Isolated midgut |
| Settore Scientifico Disciplinare: | Settore BIO/09 - Fisiologia |
| Data di pubblicazione: | 1997 |
| Rivista: | |
| Tipologia: | Article (author) |
| Digital Object Identifier (DOI): | http://dx.doi.org/10.1007/s002329900284 |
| Appare nelle tipologie: | 01 - Articolo su periodico |
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