This paper describes a quantitative assay of 2-(2-thiophenecarboxy)benzoic acid and its systemic metabolites, namely salicylic acid and thiophenecarboxylic acid, by high-performance liquid chromatography with ultraviolet detection at 254 nm. Analytes were extracted from acidified samples with tert.-butylmethyl ether and separated with an RP-18 column (150 mm × 3 mm I.D., 5 μm particle size) with a mixture of 0.01 M potassium phosphate and methanol (70:30, v/v) at pH 3.1. The method proved to have the validation required for pharmacokinetic investigations in animals and humans.
Evaluation of 2-(2-thiophenecarboxy)benzoic acid and related active metabolites in biological samples / F. Belliardo, G. Bruno, C. La Rosa, C. Lucarelli, R. Pelloso. - In: JOURNAL OF CHROMATOGRAPHY B. BIOMEDICAL APPLICATIONS. - ISSN 0378-4347. - 573:1(1992), pp. 150-153. [10.1016/0378-4347(92)80489-D]
Evaluation of 2-(2-thiophenecarboxy)benzoic acid and related active metabolites in biological samples
C. La Rosa;
1992
Abstract
This paper describes a quantitative assay of 2-(2-thiophenecarboxy)benzoic acid and its systemic metabolites, namely salicylic acid and thiophenecarboxylic acid, by high-performance liquid chromatography with ultraviolet detection at 254 nm. Analytes were extracted from acidified samples with tert.-butylmethyl ether and separated with an RP-18 column (150 mm × 3 mm I.D., 5 μm particle size) with a mixture of 0.01 M potassium phosphate and methanol (70:30, v/v) at pH 3.1. The method proved to have the validation required for pharmacokinetic investigations in animals and humans.
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