We studied the presence of surfactant protein A (Sp-A) immunoreactivity and messenger RNA in 62 normal and abnormal breast samples. Sections were immunostained with polyclonal anti-Sp-A antibody. The association between Sp-A immunoreactivity and histologic grade of 32 invasive ductal carcinomas was assessed by 3 pathologists who scored the intensity of Sp-A immunoreactivity times the percentage of tumor immunostained; individual scores were averaged, and the final scores were correlated with tumor grade, proliferative index, and expression of estrogen and progesterone receptors. Strong Sp-A immunoreactivity was present at the luminal surface of ductal epithelial cells in normal breast samples and in benign lesions; carcinomas displayed variable immunoreactivity, inversely proportional to the degree of differentiation. Sp-A messenger RNA was detected by reverse transcriptase-polymerase chain reaction in 3 of 3 normal breast samples and 9 of 9 carcinomas. The significance of Sp-A expression in breast epithelium requires further study; possibly it has a role in native host defense or epithelial differentiation.
Surfactant protein A expression in human normal and neoplastic breast epithelium / P. Braidotti, C. Cigala, D. Graziani, B. Del Curto, E. Dessy, G. Coggi, S. Bosari, G. G. Pietra. - In: AMERICAN JOURNAL OF CLINICAL PATHOLOGY. - ISSN 0002-9173. - 116:5(2001 Nov), pp. 721-728.
|Titolo:||Surfactant protein A expression in human normal and neoplastic breast epithelium|
BRAIDOTTI, PAOLA (Primo)
BOSARI, SILVANO (Penultimo)
|Parole Chiave:||RNA, Neoplasm; Pulmonary Surfactant-Associated Protein A; Humans; Breast; Breast Neoplasms; Pulmonary Surfactant-Associated Proteins; Reverse Transcriptase Polymerase Chain Reaction; Proteolipids; RNA, Messenger; Receptors, Progesterone; DNA Primers; Carcinoma, Intraductal, Noninfiltrating; Epithelium; Female; Immunoenzyme Techniques; Receptors, Estrogen; Cell Division; Pulmonary Surfactants|
|Settore Scientifico Disciplinare:||Settore MED/08 - Anatomia Patologica|
|Data di pubblicazione:||nov-2001|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1309/DU7F-XYP8-1DF6-WM9E|
|Appare nelle tipologie:||01 - Articolo su periodico|