The microbial oxidation of racemic 2-phenyl-1-propanol by Gluconobacter oxydans DSM 50049 was investigated. Whole bacterial cells were used to produce (S)-(+)-2-phenylpropanoic acid with high enantiomeric excess (E>200). A simplex sequential method was employed as an experimental design to guide the optimization process. Temperature of 26-28°C, pH 6.0-6.2, substrate concentration of 20-25 mM and agitation of 150 rpm have been found the best conditions to achieve the highest reaction rates and enantioselectivities.
Enantioselective oxidation of (RS)-2-phenyl-1-propanol to (S)-2-phenylpropanoic acid with Gluconobacter oxydans: Simplex optimization of the biotransformation / F. Molinari, R. Villa, F. Aragozzini, R. Leon, D.M.F. Prazeres. - In: TETRAHEDRON-ASYMMETRY. - ISSN 0957-4166. - 10:15(1999), pp. 3003-3009. [10.1016/S0957-4166(99)00309-2]
Enantioselective oxidation of (RS)-2-phenyl-1-propanol to (S)-2-phenylpropanoic acid with Gluconobacter oxydans: Simplex optimization of the biotransformation
F. MolinariPrimo
;R. VillaSecondo
;
1999
Abstract
The microbial oxidation of racemic 2-phenyl-1-propanol by Gluconobacter oxydans DSM 50049 was investigated. Whole bacterial cells were used to produce (S)-(+)-2-phenylpropanoic acid with high enantiomeric excess (E>200). A simplex sequential method was employed as an experimental design to guide the optimization process. Temperature of 26-28°C, pH 6.0-6.2, substrate concentration of 20-25 mM and agitation of 150 rpm have been found the best conditions to achieve the highest reaction rates and enantioselectivities.
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