Background Activation of bradykinin-mediated B2 receptor has been shown to play an important role in the onset of angioedema associated with C1 inhibitor deficiency. This finding has led to the development of novel therapeutic drugs such as the B2 receptor antagonist icatibant. However, it is unclear whether other receptors expressed on endothelial cells contribute to the release of kinins and vascular leakage in these patients. The recognition of their role may have obvious therapeutic implications. Objective Our aim was to investigate the involvement of B1 and gC1q receptors in in vitro and in vivo models of vascular leakage induced by plasma samples obtained from patients with C1 inhibitor deficiency. Methods The vascular leakage was evaluated in vitro on endothelial cells by a transwell model system and in vivo on rat mesentery microvessels by intravital microscopy. Results We observed that the attack phase plasma from C1 inhibitor–deficient patients caused a delayed fluorescein-labeled albumin leakage as opposed to the rapid effect of bradykinin, whereas remission plasma elicited a modest effect compared with control plasma. The plasma permeabilizing effect was prevented by blocking the gC1q receptor–high-molecular-weight kininogen interaction, was partially inhibited by B2 receptor or B1 receptor antagonists, and was totally prevented by the mixture of the 2 antagonists. Involvement of B1 receptor was supported by the finding that albumin leakage caused by attack phase plasma was enhanced by IL-1β and was markedly reduced by brefeldin A. Conclusion Our data suggest that both B1 receptor and gC1q receptor are involved in the vascular leakage induced by hereditary and acquired angioedema plasma.
|Titolo:||Novel pathogenic mechanism andtherapeutic approaches to angioedema associated with C1 inhibitor deficiency|
CICARDI, MARCO (Penultimo)
|Parole Chiave:||Angioedema ; C1 inhibitor ; complement ; endothelial cells ; vascular leakage ; B1R ; B2R ; gC1QR ; bradykinin ; leakage assay|
|Settore Scientifico Disciplinare:||Settore MED/09 - Medicina Interna|
|Data di pubblicazione:||dic-2009|
|Digital Object Identifier (DOI):||10.1016/j.jaci.2009.08.007|
|Appare nelle tipologie:||01 - Articolo su periodico|