We report a new dendritic cell adhesion assay, using either immature or mature dendritic cells, for identifying functional dendritic cell-specific ICAM-3-grabbing nonintegrin (DC-SIGN) inhibitors. Because immature dendritic cells are responsible for pathogen binding and invasion, this in vitro assay provides an important link between in vitro results and pathogen-based in vivo assays. Furthermore, this assay does not require laborious expression, refolding, and purification of DC-SIGN carbohydrate recognition domain or extracellular domain as receptor-based assays. The assay power evaluated with Z and Z' parameters enables screening of compound libraries and determination of IC50 values in the first stage of DC-SIGN inhibitor development.

An assay for functional dendritic cell-specific ICAM-3-grabbing nonintegrin (DC–SIGN) inhibitors of human dendritic cell adhesion / N. Obermajer, U. Švajger, M. Jeras, S. Sattin, A. Bernardi, M. Anderluh. - In: ANALYTICAL BIOCHEMISTRY. - ISSN 0003-2697. - 406:2(2010), pp. 222-229.

An assay for functional dendritic cell-specific ICAM-3-grabbing nonintegrin (DC–SIGN) inhibitors of human dendritic cell adhesion

S. Sattin;A. Bernardi
Penultimo
;
2010

Abstract

We report a new dendritic cell adhesion assay, using either immature or mature dendritic cells, for identifying functional dendritic cell-specific ICAM-3-grabbing nonintegrin (DC-SIGN) inhibitors. Because immature dendritic cells are responsible for pathogen binding and invasion, this in vitro assay provides an important link between in vitro results and pathogen-based in vivo assays. Furthermore, this assay does not require laborious expression, refolding, and purification of DC-SIGN carbohydrate recognition domain or extracellular domain as receptor-based assays. The assay power evaluated with Z and Z' parameters enables screening of compound libraries and determination of IC50 values in the first stage of DC-SIGN inhibitor development.
Anti-infectives; DC-SIGN; Dendritic cell; Dendritic cell-based assay
Settore CHIM/06 - Chimica Organica
Article (author)
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/191747
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