It has been known that haemoatoporphyrin derivative (Hpd) has a preferential distribution in tissues with high mitotic index. Furthermore, cytocidal activity of light activated Hpd within the cells has been exploited in the therapy of experimental and human cancer. It is reported here that maximum, long lasting, although reversible, inhibition of DNA synthesis was obtained in Hpd-treated lymphocytes. However, Hpd-treated lymphoid cells did not stimulate allogeneic lymphocytes in the primary mixed lymphocyte reaction (MLR) culture. Phytohaemagglutinin (PHA) stimulated murine lymphocytes treated with Hpd and exposed to laser light have shown higher susceptibility to lysis than resting, PHA unstimulated, lymphocytes. In vitro DBA/2 stimulated C57 lymphocytes, inoculated into X-irradiated BD2F1 mice, upon Hpd treatment followed by exposure to light, did not cause a lethal graft vs. host reaction (GVHR).

Haematoporphyrin-treated murine lymphocytes: in vitro inhibition of DNA synthesis and light-mediated inactivation of cells responsible for GVHR / G. Canti, O. Marelli, L. Ricci, A. Nicolin. - In: PHOTOCHEMISTRY AND PHOTOBIOLOGY. - ISSN 0031-8655. - 34:5(1981 Nov), pp. 589-594.

Haematoporphyrin-treated murine lymphocytes: in vitro inhibition of DNA synthesis and light-mediated inactivation of cells responsible for GVHR

G. Canti
Primo
;
O. Marelli
Secondo
;
A. Nicolin
Ultimo
1981-11

Abstract

It has been known that haemoatoporphyrin derivative (Hpd) has a preferential distribution in tissues with high mitotic index. Furthermore, cytocidal activity of light activated Hpd within the cells has been exploited in the therapy of experimental and human cancer. It is reported here that maximum, long lasting, although reversible, inhibition of DNA synthesis was obtained in Hpd-treated lymphocytes. However, Hpd-treated lymphoid cells did not stimulate allogeneic lymphocytes in the primary mixed lymphocyte reaction (MLR) culture. Phytohaemagglutinin (PHA) stimulated murine lymphocytes treated with Hpd and exposed to laser light have shown higher susceptibility to lysis than resting, PHA unstimulated, lymphocytes. In vitro DBA/2 stimulated C57 lymphocytes, inoculated into X-irradiated BD2F1 mice, upon Hpd treatment followed by exposure to light, did not cause a lethal graft vs. host reaction (GVHR).
Mice, Inbred Strains; Animals; Mice; Lymphocytes; Hematoporphyrins; Male; Female; DNA Replication; Graft vs Host Reaction
Settore BIO/14 - Farmacologia
Article (author)
File in questo prodotto:
Non ci sono file associati a questo prodotto.
Pubblicazioni consigliate

Caricamento pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/191619
Citazioni
  • ???jsp.display-item.citation.pmc??? 1
  • Scopus 31
  • ???jsp.display-item.citation.isi??? ND
social impact