Mice bearing the MS-2 fibrosarcoma were administered 0.25, 0.5 or 1 mg kg(-1) body weight (b.w.) of sulphonated aluminium phthalocyanine (AlS(2)Pc) (with average degree of sulphonation of 2.1), and time-gated fluorescence images were acquired up to 6 h after the injection. Different excitation wavelengths (610, 650 and 670 nm) were tested. Red light excitation and 3 ns delayed detection allow one to minimize natural fluorescence and scattered laser light, respectively. The best conditions for tumour detection are reached under either 650 or 670 nm Excitation, 2-4 h after the administration of either 0.5 or 1 mg kg(-1) b.w. of AlS(2)Pc. In these situations, the average fluorescence contrast between tumour area and surrounding healthy tissue is > 2, providing a clear identification of the pathological region. However, tumour localization is possible even after the injection of 0.25 mg kg(-1) b.w. of sensitizer. In conclusion, under low power excitation (< 100MuW cm(-2)), the technique allows real time detection of an intradermal tumour with good contrast.

Tumour visualization in a murine model by time-delayed fluorescence of sulphonated aluminium phthalocyanine / R. Cubeddu, G. Canti, P. Taroni, G. Valentini. - In: LASERS IN MEDICAL SCIENCE. - ISSN 0268-8921. - 12:3(1997 Oct), pp. 200-208.

Tumour visualization in a murine model by time-delayed fluorescence of sulphonated aluminium phthalocyanine

G. Canti
Secondo
;
1997-10

Abstract

Mice bearing the MS-2 fibrosarcoma were administered 0.25, 0.5 or 1 mg kg(-1) body weight (b.w.) of sulphonated aluminium phthalocyanine (AlS(2)Pc) (with average degree of sulphonation of 2.1), and time-gated fluorescence images were acquired up to 6 h after the injection. Different excitation wavelengths (610, 650 and 670 nm) were tested. Red light excitation and 3 ns delayed detection allow one to minimize natural fluorescence and scattered laser light, respectively. The best conditions for tumour detection are reached under either 650 or 670 nm Excitation, 2-4 h after the administration of either 0.5 or 1 mg kg(-1) b.w. of AlS(2)Pc. In these situations, the average fluorescence contrast between tumour area and surrounding healthy tissue is > 2, providing a clear identification of the pathological region. However, tumour localization is possible even after the injection of 0.25 mg kg(-1) b.w. of sensitizer. In conclusion, under low power excitation (< 100MuW cm(-2)), the technique allows real time detection of an intradermal tumour with good contrast.
Settore BIO/14 - Farmacologia
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/191579
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