A subline of the human B cell lymphoma DHL-4, grown in the artificial serum-free medium HB101, displayed a resistant phenotype to the activity of an antisense oligodeoxynucleotide (aODN) effective on the parental DHL-4 line. It was found that the cellular uptake of the 18mer aODN in the two cell lines was almost the same. In contrast, the unresponsive subline DHL-4r degraded the aODN very efficiently, in contrast to the stability of aODN inside cells of the parental DHL-4 line. Activation of the degrading 'machinery' combined with selective properties of the artificial medium may be responsible for the loss of responsiveness to aODN.

Oligonucleotide degradation contributes to resistance to antisense compounds / A. Ryte, S. Morelli, M. Mazzei, A. Alama, P. Franco, G. Canti, A.N. Nicolin. - In: ANTI-CANCER DRUGS. - ISSN 0959-4973. - 4:2(1993 Apr), pp. 197-200.

Oligonucleotide degradation contributes to resistance to antisense compounds

P. Franco;G. Canti
Penultimo
;
A.N. Nicolin
Ultimo
1993-04

Abstract

A subline of the human B cell lymphoma DHL-4, grown in the artificial serum-free medium HB101, displayed a resistant phenotype to the activity of an antisense oligodeoxynucleotide (aODN) effective on the parental DHL-4 line. It was found that the cellular uptake of the 18mer aODN in the two cell lines was almost the same. In contrast, the unresponsive subline DHL-4r degraded the aODN very efficiently, in contrast to the stability of aODN inside cells of the parental DHL-4 line. Activation of the degrading 'machinery' combined with selective properties of the artificial medium may be responsible for the loss of responsiveness to aODN.
antisense oligodeoxynucleotides; cellular uptake; resistant cells; stability
Settore BIO/14 - Farmacologia
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/191454
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