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IRIS è il sistema di gestione integrata dei dati della ricerca (persone, progetti, pubblicazioni, attività) adottato dall'Università degli Studi di Milano.
AIR nasce in UNIMI nel 2006 con lo scopo di raccogliere, documentare e conservare le informazioni sulla produzione scientifica dell'Università degli Studi di Milano e costituisce di fatto l'Anagrafe della ricerca dell'Ateneo.
EnglishCloricromene decreases myocardial infarct size after ischemic-reperfusion injury in vivo, and it has been suggested that this is due to inhibition of tumor necrosis factor-α (TNF-α). The purpose of this work was to characterize the mechanism of cloricromene-induced inhibition of TNF-α in rat macrophages. Cloricromene inhibited lipopolysaccharide-induced TNF-α release in a dose-dependent manner (IC 50 = 5.9 ± 0.8 μM). This was not due to cytotoxicity, as cloricromene was well tolerated up to 500 μM. Cloricromene inhibited lipopolysaccharide-induced expression of TNF-α mRNA, which suggests a pre-transcriptional effect. We then investigated the early signal transduction pathway triggered by lipopolysaccharide. The binding of lipopolysaccharide to its receptor CD14 activates protein kinase C and nuclear factor-κB (NF-κB). Cloricromene inhibited NF-κB activation in a dose-dependent manner, but affected protein kinase C translocation only slightly. We then established that cloricromene inhibited lipopolysaccharide-induced cellular oxidative activity, which is important for NF-κB activation. Our results show that cloricromene interferes with the early signal transduction pathway triggered by lipopolysaccharide.
Cloricromene, a semi-synthetic coumarin derivative, inhibits tumor necrosis factor-α production at a pre-transcriptional level / E. Corsini, L. Lucchi, M. Binaglia, B. Viviani, C. Bevilacqua, G. Monastra, M. Marinovich, C.L. Galli. - In: EUROPEAN JOURNAL OF PHARMACOLOGY. - ISSN 0014-2999. - 418:3(2001), pp. 231-237.
| Titolo: | Cloricromene, a semi-synthetic coumarin derivative, inhibits tumor necrosis factor-α production at a pre-transcriptional level | |
| Autori: | CORSINI, EMANUELA (Primo) LUCCHI, LAURA (Secondo) MARINOVICH, MARINA (Penultimo) GALLI, CORRADO LODOVICO (Ultimo) | |
| Settore Scientifico Disciplinare: | Settore BIO/14 - Farmacologia | |
| Data di pubblicazione: | 2001 | |
| Rivista: | ||
| Tipologia: | Article (author) | |
| Digital Object Identifier (DOI): | http://dx.doi.org/10.1016/S0014-2999(01)00910-4 | |
| Appare nelle tipologie: | 01 - Articolo su periodico |
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