Time-resolved reflectance was used to measure the absorption spectrum of hematoporphyrin derivative (HpD) in vivo in a murine tumor model. Reflectance measurements were performed in the 600-640 nm range on mice bearing the L1210 leukemia. Then the animals were administered 25 mg/kg body weight of HpD intraperitoneally. One hour later the reflectance measurements were repeated. Fitting of the data using the diffusion theory allowed assessment of the absorption coefficient before and after the administration. As a difference between the latter and the former data, the in vivo absorption spectrum of HpD was evaluated. Maximum absorption was measured at 620-625 nm. Similar spectral behavior was obtained for HpD in solution in the presence of low-density lipoproteins.
Absorption spectrum of hematoporphyrin derivative in vivo in a murine tumor model / R. Cubeddu, G. Canti, M. Musolino, A. Pifferi, P. Taroni, G. Valentini. - In: PHOTOCHEMISTRY AND PHOTOBIOLOGY. - ISSN 0031-8655. - 60:6(1994 Dec), pp. 582-585.
|Titolo:||Absorption spectrum of hematoporphyrin derivative in vivo in a murine tumor model|
CANTI, GIANFRANCO (Secondo)
|Parole Chiave:||Animals; Spectrum Analysis; Disease Models, Animal; Mice; Hematoporphyrin Derivative; Mice, Inbred BALB C; Mice, Inbred DBA; Leukemia L1210|
|Settore Scientifico Disciplinare:||Settore BIO/14 - Farmacologia|
|Data di pubblicazione:||dic-1994|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1111/j.1751-1097.1994.tb05151.x|
|Appare nelle tipologie:||01 - Articolo su periodico|