The rate of synthesis of poly(A) on a ply(dT) template by Bacillus subtilis RNA polymerase is a function of ATP concentration and is expressed as a sigmoidal curve. The addition of millimolar concentration of AMP to low concentrations of ATP stimulates synthesis of poly(A) twenty fold and raises the rate of synthesis to the levels obtained at high ATP concentrations. The reaction is completely dependent upon the presence of poly(dT) and requires the complementary mononucleotide. Stimulation of poly(A) synthesis by AMP is more evident with the holoenzyme. Analysis of poly(A) products by acrylamide gels showed that the poly(A) synthesized in the presence of AMP has an higher molecular weight than poly(A) synthesized in the absence of AMP.
Stimulation of poly(dT) transcription by Bacillus subtilis RNA polymerase in the presence of adenosine monophosphate / G. Badaracco, P. Plevani, G. Cassani. - In: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. - ISSN 0006-291X. - 99:1(1981 Mar 16), pp. 23-9-29.
|Titolo:||Stimulation of poly(dT) transcription by Bacillus subtilis RNA polymerase in the presence of adenosine monophosphate|
PLEVANI, PAOLO (Secondo)
|Parole Chiave:||Poly A; Adenosine Monophosphate; Bacterial Proteins; Bacillus subtilis; Electrophoresis, Polyacrylamide Gel; Transcription, Genetic; Adenosine Triphosphate; Poly T; DNA-Directed RNA Polymerases; Polydeoxyribonucleotides|
|Settore Scientifico Disciplinare:||Settore BIO/11 - Biologia Molecolare|
|Data di pubblicazione:||16-mar-1981|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1016/0006-291X(81)91707-1|
|Appare nelle tipologie:||01 - Articolo su periodico|