Teicoplanin, although more active than vancomycin [by minimum inhibitory concentration (MIC)], produces smaller inhibition zones in sensitivity testing with 30-μg disks. Our data support the hypothesis that this is due to lower diffusion of teicoplanin in agar media. After 6 hr of incubation, ≈70% of vancomycin, but only 20% of teicoplanin entered the agar from a paper disk charged with 30 μg of antibiotic. This is due to a difference between the diffusion coefficients: 0.47 mm2/hr for teicoplanin and 0.72 mm2/hr for vancomycin. With the methodology used in this work, it is possible to calculate the range of concentrations of the antibiotic occurring at times likely to include the critical time-the time when the inhibition zone is formed-of most strains at any given distance from the reservoir. One can thus estimate the breakpoint diameter for a given MIC breakpoint; for example, an MIC breakpoint of ≤4 μg/ml would correspond to a ≥15-mm breakpoint diameter for vancomycin (30-μg disk) and a ≥13-mm breakpoint diameter for teicoplanin (30-μg disk).

Diffusion of teicoplanin and vancomycin in agar / L. A. Cavenaghi, E. Biganzoli, A. Danese, F. Parenti. - In: DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE. - ISSN 0732-8893. - 15:3(1992), pp. 253-258.

Diffusion of teicoplanin and vancomycin in agar

E. Biganzoli
Secondo
;
1992

Abstract

Teicoplanin, although more active than vancomycin [by minimum inhibitory concentration (MIC)], produces smaller inhibition zones in sensitivity testing with 30-μg disks. Our data support the hypothesis that this is due to lower diffusion of teicoplanin in agar media. After 6 hr of incubation, ≈70% of vancomycin, but only 20% of teicoplanin entered the agar from a paper disk charged with 30 μg of antibiotic. This is due to a difference between the diffusion coefficients: 0.47 mm2/hr for teicoplanin and 0.72 mm2/hr for vancomycin. With the methodology used in this work, it is possible to calculate the range of concentrations of the antibiotic occurring at times likely to include the critical time-the time when the inhibition zone is formed-of most strains at any given distance from the reservoir. One can thus estimate the breakpoint diameter for a given MIC breakpoint; for example, an MIC breakpoint of ≤4 μg/ml would correspond to a ≥15-mm breakpoint diameter for vancomycin (30-μg disk) and a ≥13-mm breakpoint diameter for teicoplanin (30-μg disk).
Settore MED/01 - Statistica Medica
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/189707
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