Lyophilized normal and anticoagulated plasmas are increasingly used in External Quality Assessment Schemes (EQAS) to assess the quality of performance of laboratories engaged in the control of oral anticoagulant therapy by means of the International Normalized Ratio (INR) system and for the standardization of the prothrombin time (PT). The main feature of the INR system is that the value measured for an individual plasma should be independent of the reagent used. This holds true only if the variable factors influencing the responsiveness of the reagents are the vitamin K-dependent clotting factors, the other factors being constant and within normal limits. We provide evidence that even a partial factor V (FV) deficiency (FV activity < 60 U/dL) in anticoagulated plasmas can be responsible for discrepancies between INR values as measured for a single lyophilized or fresh plasma by different reagents and that the discrepancy is proportional to an magnified by the International Sensitivity Index (ISI) of the reagent used. The greater the difference between ISI values, the wider the gap between INR values. Since FV is one of the most labile plasma clotting factors, its activity is likely to be lost during freeze-drying. Hence, we recommend a thorough control of the FV activity in commercial and home-made lyophilized plasmas before their use in EQAS and in the standardization of the PT.
A partial factor V deficiency in anticoagulated lyophil¬ized plasmas was identified as a cause of the International Normalized Ratio discrepancy in External Quality Assessment Scheme / A. Tripodi, V. Chantarangkul, B. Akkawat, M. Clerici, P.M. Mannucci. - In: THROMBOSIS RESEARCH. - ISSN 0049-3848. - 78:4(1995), pp. 283-292.
|Titolo:||A partial factor V deficiency in anticoagulated lyophil¬ized plasmas was identified as a cause of the International Normalized Ratio discrepancy in External Quality Assessment Scheme|
|Settore Scientifico Disciplinare:||Settore AGR/16 - Microbiologia Agraria|
Settore MED/09 - Medicina Interna
Settore MED/15 - Malattie del Sangue
|Data di pubblicazione:||1995|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1016/0049-3848(95)00061-U|
|Appare nelle tipologie:||01 - Articolo su periodico|