Simultaneous addition of diamide (azodicarboxylic acid-bis-dimethylamide, DIA), a SH-oxidizing agent, and collagen causes a deaggregation of otherwise irreversibly aggregating platelets. Thromboxane B2 (TXB2) and 12-HE-TE formation is inhibited depending on the concentration ratio between collagen and DIA. Thus, at 0.25 mM DIA and 20 micrograms/ml collagen neither TXB2 nor 12-HETE were measurable, but a full scale reversible aggregation is induced. Deaggregation is further attained by adding DIA to collagen-induced aggregates at a time, when maximum amplitude has been achieved. Investigation of arachidonic acid (AA) metabolites under these conditions revealed no influence of DIA on AA metabolism. Therefore, AA metabolization seems to play a minor role in collagen-induced aggregation and DIA-induced deaggregation. Polymerization of certain cytoskeletal proteins of the platelets, after addition of DIA, parallels DIA-induced deaggregation. DIA inhibits endogenous AA release, probably by interaction with platelet plasma membrane. DIA seems to inhibit the release of the alpha-granula protein thrombospondin
Influence of diamide on aggregation, cytoskeletal proteins and arachidonic acid metabolism in human platelets / D. Caruso, G. Galli, U. Till, P. Spangenberg, W. Lösche, R. Paoletti. - In: THROMBOSIS RESEARCH. - ISSN 0049-3848. - 36:1(1984), pp. 9-16.
|Titolo:||Influence of diamide on aggregation, cytoskeletal proteins and arachidonic acid metabolism in human platelets|
CARUSO, DONATELLA (Primo)
|Settore Scientifico Disciplinare:||Settore BIO/10 - Biochimica|
|Data di pubblicazione:||1984|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1016/0049-3848(84)90371-2|
|Appare nelle tipologie:||01 - Articolo su periodico|