A technique suitable to immobilize enzymes by photografting of acrylic monomers or prepolymers onto microporous polymer substrates has been studied in this work. Some preliminary kinetic experiments have then been carried out on photosynthetic membranes immobilizing catalase as model enzyme, both in conditions of internal diffusion controlled reaction and of ultrafiltration through the membranes, in order to get information about the efficiency of the immobilized biocatalyst in this system.
Immobilization of biocatalysts by photochemically grafted membranes: some studies with catalase as model system / I.R. Bellobono, E. Selli, A. Polissi, F. Muffato. - In: BIOTECHNOLOGY AND BIOENGINEERING. - ISSN 0006-3592. - 35:6(1990), pp. 646-649. [10.1002/bit.260350610]
Immobilization of biocatalysts by photochemically grafted membranes: some studies with catalase as model system
I.R. Bellobono;E. Selli;A. Polissi;
1990
Abstract
A technique suitable to immobilize enzymes by photografting of acrylic monomers or prepolymers onto microporous polymer substrates has been studied in this work. Some preliminary kinetic experiments have then been carried out on photosynthetic membranes immobilizing catalase as model enzyme, both in conditions of internal diffusion controlled reaction and of ultrafiltration through the membranes, in order to get information about the efficiency of the immobilized biocatalyst in this system.
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