The cloned DNA polymerase I gene has been used to map the POL1 locus on the left arm of chromosome XIV, between MET4 and TOP2. Temperature-sensitive mutants in POL1 have been obtained by in vitro mutagenesis of the cloned gene and in vivo replacement of the wild-type allele with the mutated copy. Physiological and biochemical characterization of one temperature-sensitive mutant (pol1-1) shows that cells shifted to the non-permissive temperature can complete one round of cell division and DNA replication before they arrest. Analysis of DNA polymerase I in crude extracts and in partially purified preparations indicates that the pol1-1 mutation results in a conformational change and affects the stability of the DNA primase-polymerase complex.
|Titolo:||Genetic mapping of the Saccharomyces cerevisiae DNA polymerase I gene and characterization of a pol1 temperature-sensitive mutant altered in DNA primase-polymerase complex stability|
|Parole Chiave:||DNA Primase; DNA Polymerase I; Genes, Fungal; Temperature; RNA Nucleotidyltransferases; Chromosome Mapping; Mutation; DNA Replication; Saccharomyces cerevisiae|
|Settore Scientifico Disciplinare:||Settore BIO/11 - Biologia Molecolare|
|Data di pubblicazione:||giu-1988|
|Digital Object Identifier (DOI):||10.1007/BF00330850|
|Appare nelle tipologie:||01 - Articolo su periodico|