The cloned DNA polymerase I gene has been used to map the POL1 locus on the left arm of chromosome XIV, between MET4 and TOP2. Temperature-sensitive mutants in POL1 have been obtained by in vitro mutagenesis of the cloned gene and in vivo replacement of the wild-type allele with the mutated copy. Physiological and biochemical characterization of one temperature-sensitive mutant (pol1-1) shows that cells shifted to the non-permissive temperature can complete one round of cell division and DNA replication before they arrest. Analysis of DNA polymerase I in crude extracts and in partially purified preparations indicates that the pol1-1 mutation results in a conformational change and affects the stability of the DNA primase-polymerase complex.
Genetic mapping of the Saccharomyces cerevisiae DNA polymerase I gene and characterization of a pol1 temperature-sensitive mutant altered in DNA primase-polymerase complex stability / G. Lucchini, C. Mazza, E. Scacheri, P. Plevani. - In: MOLECULAR AND GENERAL GENETICS. - ISSN 0026-8925. - 212:3(1988 Jun), pp. 459-65-465.
Titolo: | Genetic mapping of the Saccharomyces cerevisiae DNA polymerase I gene and characterization of a pol1 temperature-sensitive mutant altered in DNA primase-polymerase complex stability |
Autori: | |
Parole Chiave: | DNA Primase; DNA Polymerase I; Genes, Fungal; Temperature; RNA Nucleotidyltransferases; Chromosome Mapping; Mutation; DNA Replication; Saccharomyces cerevisiae |
Settore Scientifico Disciplinare: | Settore BIO/11 - Biologia Molecolare |
Data di pubblicazione: | giu-1988 |
Rivista: | |
Tipologia: | Article (author) |
Digital Object Identifier (DOI): | http://dx.doi.org/10.1007/BF00330850 |
Appare nelle tipologie: | 01 - Articolo su periodico |