A cohort of 39 vertically infected children (class N, A, B, and C of the CDC HIV classification for pediatric infection) was studied by virus isolation and non-syncytium inducing (NSI)/syncytium inducing (SI) HIV-1 phenotype evaluation. The HIV-1 isolates were recovered from PBMCs and the MT-2 cell line was used to perform the syncytium assay. HIV-1 could be isolated in 34 of 39 (87%) infected children, regardless of the clinical and immunological stage of the disease. Class N and A subjects harbored exclusively NSI strains, whereas the SI phenotype was detected in two of eight class B and five of nine class C patients. All of the SI variants were observed in severely CD4-depleted children (class 3 patients). The capability of pediatric HIV-1 isolates to induce a cytopathic effect is associated with the clinical status and the degree of CD4 depletion. These data suggest that the biological properties of HIV-1 isolates in children do not differ from those observed in adults, and that viral phenotype strictly correlates with disease progression in vertically infected children.
|Titolo:||HIV type 1 phenotype correlates with the stage of infection in vertically infected children|
|Parole Chiave:||Infectious Disease Transmission, Vertical; Coculture Techniques; Humans; Child; HIV Core Protein p24; HIV-1; Child, Preschool; Infant; Phenotype; HIV Infections; Cohort Studies; Cytopathogenic Effect, Viral; Cell Line|
|Settore Scientifico Disciplinare:||Settore MED/17 - Malattie Infettive|
|Data di pubblicazione:||1-set-1996|
|Digital Object Identifier (DOI):||10.1089/aid.1996.12.1247|
|Appare nelle tipologie:||01 - Articolo su periodico|