Electrical field stimulation caused frequency-dependent relaxations in precontracted strips of hamster proximal urethra, which were attenuated by L-N(G)-nitroarginine methyl ester (10(-4) M) and completely blocked by tetrodotoxin (10(-6) M). Strips of hamster urethra devoid of urothelium showed reduced relaxant responses to electrical field stimulation which were abolished by L-N(G)-nitroarginine methyl ester (10(-4) M). Western blot analysis showed the presence of a constitutive endothelial nitric oxide synthase in the urothelial layer, suggesting that urothelium may release nitric oxide in response to electrical field stimulation and that this release is blocked by tetrodotoxin. It is suggested that the urothelium may contribute to relaxations of the smooth muscle of hamster urethra produced by nerve stimulation.

Presence of constitutive endothelial nitric oxide synthase immunoreactivity in urothelial cells of hamster proximal urethra / C. Pinna, I. Eberini, L. Puglisi, G. Burnstock. - In: EUROPEAN JOURNAL OF PHARMACOLOGY. - ISSN 0014-2999. - 367:1(1999 Feb 12), pp. 85-89. [10.1016/S0014-2999(98)00981-9]

Presence of constitutive endothelial nitric oxide synthase immunoreactivity in urothelial cells of hamster proximal urethra

C. Pinna;I. Eberini;L. Puglisi;
1999-02-12

Abstract

Electrical field stimulation caused frequency-dependent relaxations in precontracted strips of hamster proximal urethra, which were attenuated by L-N(G)-nitroarginine methyl ester (10(-4) M) and completely blocked by tetrodotoxin (10(-6) M). Strips of hamster urethra devoid of urothelium showed reduced relaxant responses to electrical field stimulation which were abolished by L-N(G)-nitroarginine methyl ester (10(-4) M). Western blot analysis showed the presence of a constitutive endothelial nitric oxide synthase in the urothelial layer, suggesting that urothelium may release nitric oxide in response to electrical field stimulation and that this release is blocked by tetrodotoxin. It is suggested that the urothelium may contribute to relaxations of the smooth muscle of hamster urethra produced by nerve stimulation.
Animals ; Arginine ; Urothelium ; Tetrodotoxin ; Enzyme Inhibitors ; Electric Stimulation ; Blotting, Western ; NG-Nitroarginine Methyl Ester ; Nitric Oxide Synthase Type III ; Nitric Oxide Synthase ; Muscle, Smooth ; Mesocricetus ; Muscle Contraction ; Muscle Relaxation ; Male ; Urethra ; Cricetinae
Settore BIO/15 - Biologia Farmaceutica
Settore BIO/10 - Biochimica
Settore BIO/14 - Farmacologia
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/188944
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