In two-component phosphatidylcholine bilayers with coexisting liquid and P-beta' gel phases, the distribution between phases of low concentrations of glycosphingolipids can be determined by freeze-etch electron microscopy after labeling the glycolipid with a suitable protein. We have found that the distribution depends upon the glycosphingolipid species (Rock, P. et al., (1991) Biochemistry 30, 19-25). Using this technique with cholera toxin as the protein label and bilayers formed from dipalmitoyl- and dielaidoylphosphatidylcholine (1:1) containing < 1 mol% GM1, we have studied the distribution of a family of GM1 homologues differing in the acyl chain and sphingoid base moieties. The GM1 preference for the P-beta' ripple phase decreases with decreasing acyl chain length and increasing unsaturation. GM1 with either a C18:1 or C20:1 sphingoid base shows similar distributions in liquid and gel phases. When the molecules are preferentially found in the P-beta' phase, they are positioned along unique loci in both Lambda and Lambda/2 forms of the ripple structure. This localization and acyl chain dependence reflect the volume, shape and localization of molecular packing defects in the P-beta' phase. The ganglioside inclusions stabilize the P beta' phase and form compositional domains of unique topography.

GEL PHASE PREFERENCE OF GANGLIOSIDE GM1 AT LOW CONCENTRATION IN 2-COMPONENT, 2-PHASE PHOSPHATIDYLCHOLINE BILAYERS DEPENDS UPON THE CERAMIDE MOIETY / P. PALESTINI, M. ALLIETTA, S. SONNINO, G. TETTAMANTI, T. THOMPSON, T. TILLACK. - In: BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES. - ISSN 0005-2736. - 1235:2(1995), pp. 221-230.

GEL PHASE PREFERENCE OF GANGLIOSIDE GM1 AT LOW CONCENTRATION IN 2-COMPONENT, 2-PHASE PHOSPHATIDYLCHOLINE BILAYERS DEPENDS UPON THE CERAMIDE MOIETY

S. SONNINO;G. TETTAMANTI;
1995

Abstract

In two-component phosphatidylcholine bilayers with coexisting liquid and P-beta' gel phases, the distribution between phases of low concentrations of glycosphingolipids can be determined by freeze-etch electron microscopy after labeling the glycolipid with a suitable protein. We have found that the distribution depends upon the glycosphingolipid species (Rock, P. et al., (1991) Biochemistry 30, 19-25). Using this technique with cholera toxin as the protein label and bilayers formed from dipalmitoyl- and dielaidoylphosphatidylcholine (1:1) containing < 1 mol% GM1, we have studied the distribution of a family of GM1 homologues differing in the acyl chain and sphingoid base moieties. The GM1 preference for the P-beta' ripple phase decreases with decreasing acyl chain length and increasing unsaturation. GM1 with either a C18:1 or C20:1 sphingoid base shows similar distributions in liquid and gel phases. When the molecules are preferentially found in the P-beta' phase, they are positioned along unique loci in both Lambda and Lambda/2 forms of the ripple structure. This localization and acyl chain dependence reflect the volume, shape and localization of molecular packing defects in the P-beta' phase. The ganglioside inclusions stabilize the P beta' phase and form compositional domains of unique topography.
AGM1; asialo-GM1; DEPC; dielaidoylphosphatidylcholine; dipalmitoylphosphatidylcholine; DPPC; Electron microscopy; fluorescence recovery after photobleaching; FRAP; Freeze-etch; Galβ1 → 3GalNAcβ1 → 4Gal(3← 2αNeu5Ac)β1 → 4G1cβ1 → 1Cer; Ganglioside; Glycosphingolipid; glycosylphosphatidylinositol; GM1; GPI; high-performance liquid chromatography; HPLC; Liposome; tetrahydrofuran; THF; thin-layer chromatography; TLC
Settore BIO/10 - Biochimica
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/188800
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