Previous studies have shown that sphingolipids mag be enriched in caveolae, plasmalemmal invaginations implicated in endocytosis and signal transduction. We synthesised a radio-labeled derivative of ganglioside GM1 bearing a photo-reactive cross-linker at the end of its acyl chain, After insertion in the plasma membrane of cultured A431 or MDCK cells and photoactivation, the main protein cross-linked by the GM1 derivative was VIP21-caveolin, an essential structural component of caveolae, This result shows close proximity between GM1 molecules and VIP21-caveolin in the caveolar membrane and strongly implicates sphingolipid segregation in the biogenesis of caveolae.
A PHOTO-REACTIVE DERIVATIVE OF GANGLIOSIDE GM1 SPECIFICALLY CROSS-LINKS VIP21-CAVEOLIN ON THE CELL-SURFACE / A. FRA, M. MASSERINI, P. PALESTINI, S. SONNINO, K. SIMONS. - In: FEBS LETTERS. - ISSN 0014-5793. - 375:1-2(1995), pp. 11-14.
A PHOTO-REACTIVE DERIVATIVE OF GANGLIOSIDE GM1 SPECIFICALLY CROSS-LINKS VIP21-CAVEOLIN ON THE CELL-SURFACE
S. SONNINOPenultimo
;
1995
Abstract
Previous studies have shown that sphingolipids mag be enriched in caveolae, plasmalemmal invaginations implicated in endocytosis and signal transduction. We synthesised a radio-labeled derivative of ganglioside GM1 bearing a photo-reactive cross-linker at the end of its acyl chain, After insertion in the plasma membrane of cultured A431 or MDCK cells and photoactivation, the main protein cross-linked by the GM1 derivative was VIP21-caveolin, an essential structural component of caveolae, This result shows close proximity between GM1 molecules and VIP21-caveolin in the caveolar membrane and strongly implicates sphingolipid segregation in the biogenesis of caveolae.
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