Previous studies have shown that sphingolipids mag be enriched in caveolae, plasmalemmal invaginations implicated in endocytosis and signal transduction. We synthesised a radio-labeled derivative of ganglioside GM1 bearing a photo-reactive cross-linker at the end of its acyl chain, After insertion in the plasma membrane of cultured A431 or MDCK cells and photoactivation, the main protein cross-linked by the GM1 derivative was VIP21-caveolin, an essential structural component of caveolae, This result shows close proximity between GM1 molecules and VIP21-caveolin in the caveolar membrane and strongly implicates sphingolipid segregation in the biogenesis of caveolae.
A PHOTO-REACTIVE DERIVATIVE OF GANGLIOSIDE GM1 SPECIFICALLY CROSS-LINKS VIP21-CAVEOLIN ON THE CELL-SURFACE / A. FRA, M. MASSERINI, P. PALESTINI, S. SONNINO, K. SIMONS. - In: FEBS LETTERS. - ISSN 0014-5793. - 375:1-2(1995), pp. 11-14.
Titolo: | A PHOTO-REACTIVE DERIVATIVE OF GANGLIOSIDE GM1 SPECIFICALLY CROSS-LINKS VIP21-CAVEOLIN ON THE CELL-SURFACE |
Autori: | SONNINO, SANDRO (Penultimo) |
Settore Scientifico Disciplinare: | Settore BIO/10 - Biochimica |
Data di pubblicazione: | 1995 |
Rivista: | |
Tipologia: | Article (author) |
Digital Object Identifier (DOI): | http://dx.doi.org/10.1016/0014-5793(95)95228-O |
Appare nelle tipologie: | 01 - Articolo su periodico |