The conformational dynamics of the carbohydrate headgroup of ganglioside GD1a, NeuAc alpha 2-->3Gal beta 1-->3GalNAc beta 1-->4[NeuAc alpha 2-->3]Gal beta 1-->4Glc beta 1-1Cer, anchored in a perdeuterated dodecylphosphocholine micelle in aqueous solution, were probed by high resolution NMR spectroscopy. The observed H-1/H-1 NOE interactions revealed conformational averaging of the terminal NeuAc alpha 2-->3Gal and Gal beta 1-->3GalNAc glycosidic linkages. The pronounced flexibility of this trisaccharide moiety was substantiated further by two-dimensional proton-detected C-13 T-1, T-1p and H-1/C-13 NOE measurements. The anchoring effect of the micelle allowed the detection of conformational fluctuations of the headgroup on the time scale of a few hundred picoseconds. NMR experiments performed on the GD1a/DPC micelles in H2O at low temperatures permitted the observation of hydroxyl proton resonances, contributing valuable conformational information.
CARBOHYDRATE DYNAMICS AT A MICELLAR SURFACE - GD1A HEADGROUP TRANSFORMATIONS REVEALED BY NMR-SPECTROSCOPY / L. POPPE, H. VANHALBEEK, D. ACQUOTTI, S. SONNINO. - In: BIOPHYSICAL JOURNAL. - ISSN 0006-3495. - 66:5(1994), pp. 1642-1652.
Titolo: | CARBOHYDRATE DYNAMICS AT A MICELLAR SURFACE - GD1A HEADGROUP TRANSFORMATIONS REVEALED BY NMR-SPECTROSCOPY |
Autori: | SONNINO, SANDRO (Ultimo) |
Settore Scientifico Disciplinare: | Settore BIO/10 - Biochimica |
Data di pubblicazione: | 1994 |
Rivista: | |
Tipologia: | Article (author) |
Digital Object Identifier (DOI): | http://dx.doi.org/10.1016/S0006-3495(94)80956-7 |
Appare nelle tipologie: | 01 - Articolo su periodico |