The conformational dynamics of the carbohydrate headgroup of ganglioside GD1a, NeuAc alpha 2-->3Gal beta 1-->3GalNAc beta 1-->4[NeuAc alpha 2-->3]Gal beta 1-->4Glc beta 1-1Cer, anchored in a perdeuterated dodecylphosphocholine micelle in aqueous solution, were probed by high resolution NMR spectroscopy. The observed H-1/H-1 NOE interactions revealed conformational averaging of the terminal NeuAc alpha 2-->3Gal and Gal beta 1-->3GalNAc glycosidic linkages. The pronounced flexibility of this trisaccharide moiety was substantiated further by two-dimensional proton-detected C-13 T-1, T-1p and H-1/C-13 NOE measurements. The anchoring effect of the micelle allowed the detection of conformational fluctuations of the headgroup on the time scale of a few hundred picoseconds. NMR experiments performed on the GD1a/DPC micelles in H2O at low temperatures permitted the observation of hydroxyl proton resonances, contributing valuable conformational information.

CARBOHYDRATE DYNAMICS AT A MICELLAR SURFACE - GD1A HEADGROUP TRANSFORMATIONS REVEALED BY NMR-SPECTROSCOPY / L. POPPE, H. VANHALBEEK, D. ACQUOTTI, S. SONNINO. - In: BIOPHYSICAL JOURNAL. - ISSN 0006-3495. - 66:5(1994), pp. 1642-1652. [10.1016/S0006-3495(94)80956-7]

CARBOHYDRATE DYNAMICS AT A MICELLAR SURFACE - GD1A HEADGROUP TRANSFORMATIONS REVEALED BY NMR-SPECTROSCOPY

S. Sonnino
Ultimo
1994

Abstract

The conformational dynamics of the carbohydrate headgroup of ganglioside GD1a, NeuAc alpha 2-->3Gal beta 1-->3GalNAc beta 1-->4[NeuAc alpha 2-->3]Gal beta 1-->4Glc beta 1-1Cer, anchored in a perdeuterated dodecylphosphocholine micelle in aqueous solution, were probed by high resolution NMR spectroscopy. The observed H-1/H-1 NOE interactions revealed conformational averaging of the terminal NeuAc alpha 2-->3Gal and Gal beta 1-->3GalNAc glycosidic linkages. The pronounced flexibility of this trisaccharide moiety was substantiated further by two-dimensional proton-detected C-13 T-1, T-1p and H-1/C-13 NOE measurements. The anchoring effect of the micelle allowed the detection of conformational fluctuations of the headgroup on the time scale of a few hundred picoseconds. NMR experiments performed on the GD1a/DPC micelles in H2O at low temperatures permitted the observation of hydroxyl proton resonances, contributing valuable conformational information.
Settore BIO/10 - Biochimica
Article (author)
File in questo prodotto:
Non ci sono file associati a questo prodotto.
Pubblicazioni consigliate

Caricamento pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/188705
Citazioni
  • ???jsp.display-item.citation.pmc??? 8
  • Scopus 71
  • ???jsp.display-item.citation.isi??? 71
social impact