The small intestine of 51 calves was examined for the presence of K99+ Escherichia coli by means of both an immunoperoxidase procedure performed on paraffin sections and by the slide agglutination test after isolation. Twelve cases resulted immunoperoxidase positive (23.5%) and 8 of them were also agglutination positive. Results of the 2 diagnostic tests agreed in 46 cases (90.2%). In immunoperoxidase positive sections a thick layer of immunoreactive bacteria was seen on the luminal surface of the enterocytes. Post-mortem autolysis or prolonged fixation did not seem to affect the immunoperoxidase reactivity of the sample.
|Titolo:||The diagnostic value of immunoperoxidase in detecting K99+ Escherichia coli on histological sections|
SCANZIANI, EUGENIO (Primo)
|Parole Chiave:||Cattle Diseases ; Animals ; Cattle ; Diarrhea ; Escherichia coli Infections ; Intestine, Small ; Escherichia coli ; Immunohistochemistry ; Immunoenzyme Techniques|
|Settore Scientifico Disciplinare:||Settore VET/03 - Patologia Generale e Anatomia Patologica Veterinaria|
|Data di pubblicazione:||feb-1991|
|Digital Object Identifier (DOI):||10.1111/j.1439-0450.1991.tb00840.x|
|Appare nelle tipologie:||01 - Articolo su periodico|