The small intestine of 51 calves was examined for the presence of K99+ Escherichia coli by means of both an immunoperoxidase procedure performed on paraffin sections and by the slide agglutination test after isolation. Twelve cases resulted immunoperoxidase positive (23.5%) and 8 of them were also agglutination positive. Results of the 2 diagnostic tests agreed in 46 cases (90.2%). In immunoperoxidase positive sections a thick layer of immunoreactive bacteria was seen on the luminal surface of the enterocytes. Post-mortem autolysis or prolonged fixation did not seem to affect the immunoperoxidase reactivity of the sample.
The diagnostic value of immunoperoxidase in detecting K99+ Escherichia coli on histological sections / E. Scanziani, M. Luini, L. Crippa, A. D. Nigrelli, A. Lavazza. - In: JOURNAL OF VETERINARY MEDICINE. SERIES B. - ISSN 0931-1793. - 38:1(1991 Feb), pp. 17-20.
The diagnostic value of immunoperoxidase in detecting K99+ Escherichia coli on histological sections
E. ScanzianiPrimo
;
1991
Abstract
The small intestine of 51 calves was examined for the presence of K99+ Escherichia coli by means of both an immunoperoxidase procedure performed on paraffin sections and by the slide agglutination test after isolation. Twelve cases resulted immunoperoxidase positive (23.5%) and 8 of them were also agglutination positive. Results of the 2 diagnostic tests agreed in 46 cases (90.2%). In immunoperoxidase positive sections a thick layer of immunoreactive bacteria was seen on the luminal surface of the enterocytes. Post-mortem autolysis or prolonged fixation did not seem to affect the immunoperoxidase reactivity of the sample.Pubblicazioni consigliate
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