The small intestine of 51 calves was examined for the presence of K99+ Escherichia coli by means of both an immunoperoxidase procedure performed on paraffin sections and by the slide agglutination test after isolation. Twelve cases resulted immunoperoxidase positive (23.5%) and 8 of them were also agglutination positive. Results of the 2 diagnostic tests agreed in 46 cases (90.2%). In immunoperoxidase positive sections a thick layer of immunoreactive bacteria was seen on the luminal surface of the enterocytes. Post-mortem autolysis or prolonged fixation did not seem to affect the immunoperoxidase reactivity of the sample.

The diagnostic value of immunoperoxidase in detecting K99+ Escherichia coli on histological sections / E. Scanziani, M. Luini, L. Crippa, A. D. Nigrelli, A. Lavazza. - In: JOURNAL OF VETERINARY MEDICINE. SERIES B. - ISSN 0931-1793. - 38:1(1991 Feb), pp. 17-20.

The diagnostic value of immunoperoxidase in detecting K99+ Escherichia coli on histological sections

E. Scanziani
Primo
;
1991

Abstract

The small intestine of 51 calves was examined for the presence of K99+ Escherichia coli by means of both an immunoperoxidase procedure performed on paraffin sections and by the slide agglutination test after isolation. Twelve cases resulted immunoperoxidase positive (23.5%) and 8 of them were also agglutination positive. Results of the 2 diagnostic tests agreed in 46 cases (90.2%). In immunoperoxidase positive sections a thick layer of immunoreactive bacteria was seen on the luminal surface of the enterocytes. Post-mortem autolysis or prolonged fixation did not seem to affect the immunoperoxidase reactivity of the sample.
Cattle Diseases ; Animals ; Cattle ; Diarrhea ; Escherichia coli Infections ; Intestine, Small ; Escherichia coli ; Immunohistochemistry ; Immunoenzyme Techniques
Settore VET/03 - Patologia Generale e Anatomia Patologica Veterinaria
Article (author)
File in questo prodotto:
Non ci sono file associati a questo prodotto.
Pubblicazioni consigliate

Caricamento pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/188611
Citazioni
  • ???jsp.display-item.citation.pmc??? 0
  • Scopus 0
  • ???jsp.display-item.citation.isi??? ND
social impact