Chemical modification of poly(L-lysine) by hydrogen transfer addition of N,N-dimethylacrylamide to the primary amino groups results in new water soluble derivatives with reduced cell toxicity which maintain basic properties. Kinetic studies on the addition reaction were performed using model compounds, and a reliable kinetic model was obtained which proved to be valuable also in the case of poly(L-lysine). A peculiar feature of the addition reaction of poly(L-lysine) on N,N-dimethylacrylamide is that, starting from partially protonated poly(L-lysine), the reaction stops after that all the unprotonated units originally present have reacted. Toxicological studies on modified poly(L-lysine) in comparsion with parent poly(L-lysine) showed that the modifying reaction leads to a reduction in toxicity.

A novel modification of poly(L-lysine) leading to a soluble cationic polymer with reduced toxicity and with potential as a transfection agent / F. P., K. S., E. Ranucci, D. R., G. E.. - In: MACROMOLECULAR CHEMISTRY AND PHYSICS. - ISSN 1022-1352. - 199:11(1998), pp. 2565-2575.

A novel modification of poly(L-lysine) leading to a soluble cationic polymer with reduced toxicity and with potential as a transfection agent

E. Ranucci
Ultimo
;
1998

Abstract

Chemical modification of poly(L-lysine) by hydrogen transfer addition of N,N-dimethylacrylamide to the primary amino groups results in new water soluble derivatives with reduced cell toxicity which maintain basic properties. Kinetic studies on the addition reaction were performed using model compounds, and a reliable kinetic model was obtained which proved to be valuable also in the case of poly(L-lysine). A peculiar feature of the addition reaction of poly(L-lysine) on N,N-dimethylacrylamide is that, starting from partially protonated poly(L-lysine), the reaction stops after that all the unprotonated units originally present have reacted. Toxicological studies on modified poly(L-lysine) in comparsion with parent poly(L-lysine) showed that the modifying reaction leads to a reduction in toxicity.
Settore CHIM/04 - Chimica Industriale
Article (author)
File in questo prodotto:
Non ci sono file associati a questo prodotto.
Pubblicazioni consigliate

Caricamento pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/188490
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 39
  • ???jsp.display-item.citation.isi??? 35
social impact