Five major gangliosides (GM1, GD1a, GD1b, GT1b, and GQ1b) were extracted and isolated by normal-phase HPLC from the forebrain of Sprague-Dawley rats of ages ranging from 3 days to 24 months. Each ganglioside was fractionated by reverse-phase HPLC into the molecular species carrying a single long-chain base moiety. At all ages, the C18:1 and C20:1 long-chain base species predominated, whereas the C18:0 and C20:0 ones represented 1-3% of the total. The C18:1 long-chain base species, predominant at 3 days (91-96%), diminished with age and reached, at 2 years, 73%, 65%, 61%, 59%, and 45% of the total for GD1a, GM1, GT1b, GD1b, and GQ1b, respectively. The content of the C20:1 long-chain base species, low at birth (4-9%), increased with age in all gangliosides and reached, at 2 years, 27-55% of the total. The developmental behavior of the ganglioside species containing the C18:1 long-chain base was characterized by the following: (a) a biphasic profile with a maximum around 15 days for GD1a, the most abundant ganglioside at all ages; (b) an increase until 6 months for GM1; (c) a sharp decrease until 30 days, followed by leveling for GT1b; and (d) a low, constant level for GD1b. All the ganglioside species containing the C20:1 long-chain base showed a constant increase during development, the increase being more marked in the first 30 days. The molecular species of all gangliosides carrying the C18:1 long-chain base were virtually devoid of 20:0 fatty acids and carried a higher content of 18:0 fatty acids than the corresponding C20:1 long-chain base species (average 80 versus 57%). Moreover, in the C18:1 long-chain base species, the 18:0 fatty acid content diminished with age from 89 to 72%, with a concurrent increase of 16:0 and 18:1 fatty acids, whereas the C20:1 long-chain base species had an age-constant fatty acid composition.
CHANGES IN THE CERAMIDE COMPOSITION OF RAT FOREBRAIN GANGLIOSIDES WITH AGE / P. PALESTINI, M. MASSERINI, S. SONNINO, A. GIULIANI, G. TETTAMANTI. - In: JOURNAL OF NEUROCHEMISTRY. - ISSN 0022-3042. - 54:1(1990), pp. 230-235.
|Titolo:||CHANGES IN THE CERAMIDE COMPOSITION OF RAT FOREBRAIN GANGLIOSIDES WITH AGE|
GIULIANI, ATTILIA ENRICA MARIA (Penultimo)
TETTAMANTI, GUIDO (Ultimo)
|Parole Chiave:||Age; Brain; Fatty acid; Ganglioside; Long-chain base|
|Settore Scientifico Disciplinare:||Settore BIO/10 - Biochimica|
|Data di pubblicazione:||1990|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1111/j.1471-4159.1990.tb13305.x|
|Appare nelle tipologie:||01 - Articolo su periodico|