We evaluated the ability of circulating T lymphocytes obtained from patients with systemic sclerosis (SSc) to induce the expression of tissue factor (TF) by human umbilical vein endothelial cells (HUVECs), and compared the results with those obtained from healthy controls. Nine patients with SSc and 10 sex- and age-matched healthy subjects were studied. Peripheral T lymphocytes obtained from SSc patients induced TF activity from HUVECs in a dose-dependent manner. A significant induction of endothelial TF was observed when 2 x 106 lymphocytes per well (TF values, mean ± SD: 0.34 ± 0.21U/μg of cell protein vs 0.04 ± 0.03; n = 9, p = 0.001) or 1 x 106 lymphocytes per well (0.13 ± 0.06 vs 0.04 ± 0.04; n = 8, p<0.001) were added to HUVEC cultures. Lower concentrations of T lymphocytes were ineffective. Similar results were obtained with control lymphocytes. There were no differences in endothelial TF induction between patients and controls at any lymphocyte concentration tested. Within the SSc group, there were no correlations between TF activity and clinical features or disease duration.

Endothelial tissue factor induction by T lymphocytes in systemic sclerosis / S. Della Bella, L. Mussoni, M. Molteni, S. Colli, C. Zulian, M. Camera, E. Tremoli, R. Scorza. - In: CLINICAL RHEUMATOLOGY. - ISSN 0770-3198. - 18:1(1999), pp. 38-41.

Endothelial tissue factor induction by T lymphocytes in systemic sclerosis

S. Della Bella;L. Mussoni;S. Colli;M. Camera;E. Tremoli;R. Scorza
1999

Abstract

We evaluated the ability of circulating T lymphocytes obtained from patients with systemic sclerosis (SSc) to induce the expression of tissue factor (TF) by human umbilical vein endothelial cells (HUVECs), and compared the results with those obtained from healthy controls. Nine patients with SSc and 10 sex- and age-matched healthy subjects were studied. Peripheral T lymphocytes obtained from SSc patients induced TF activity from HUVECs in a dose-dependent manner. A significant induction of endothelial TF was observed when 2 x 106 lymphocytes per well (TF values, mean ± SD: 0.34 ± 0.21U/μg of cell protein vs 0.04 ± 0.03; n = 9, p = 0.001) or 1 x 106 lymphocytes per well (0.13 ± 0.06 vs 0.04 ± 0.04; n = 8, p<0.001) were added to HUVEC cultures. Lower concentrations of T lymphocytes were ineffective. Similar results were obtained with control lymphocytes. There were no differences in endothelial TF induction between patients and controls at any lymphocyte concentration tested. Within the SSc group, there were no correlations between TF activity and clinical features or disease duration.
Endothelial cells; Lymphocytes; Systemic sclerosis; Tissue factor
Settore MED/04 - Patologia Generale
Settore MED/09 - Medicina Interna
Settore BIO/14 - Farmacologia
CLINICAL RHEUMATOLOGY
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/187826
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