When monoamine oxidase B (MAO B) from rat liver mitochondria was incubated with MPTP, methylamine was formed and MAO B was inactivated. Incubation of 1-methyl-4-phenyl-2,3-dihydropyridinium (MPDP+) with MAO B also yielded methylamine and resulted in inactivation of the enzyme. Similar rate consts. for inactivation of MAO B were obtained with 1-methyl-4-phenyl-1,4-dihydropyridine, 3-phenylglutaraldehyde (3-PG), and 2,6-dihydroxy-4-phenyltetrahydropyran, suggesting that bioalkylation of the enzyme occurs outside the active site via a species such as 3-PG.

Mechanistic studies on the MPTP inactivation of MAO B / N. Castagnoli, A. Sparatore, S. Ottoboni, L. Leung, A. Trevor. - In: INTERNATIONAL CONGRESS SERIES. - ISSN 0531-5131. - 750 (Pharmacology):(1987), pp. 875-878.

Mechanistic studies on the MPTP inactivation of MAO B

A. Sparatore
Secondo
;
1987

Abstract

When monoamine oxidase B (MAO B) from rat liver mitochondria was incubated with MPTP, methylamine was formed and MAO B was inactivated. Incubation of 1-methyl-4-phenyl-2,3-dihydropyridinium (MPDP+) with MAO B also yielded methylamine and resulted in inactivation of the enzyme. Similar rate consts. for inactivation of MAO B were obtained with 1-methyl-4-phenyl-1,4-dihydropyridine, 3-phenylglutaraldehyde (3-PG), and 2,6-dihydroxy-4-phenyltetrahydropyran, suggesting that bioalkylation of the enzyme occurs outside the active site via a species such as 3-PG.
monoamine oxidase B inactivation; MPTP and its metabolites;
Settore CHIM/08 - Chimica Farmaceutica
Article (author)
File in questo prodotto:
Non ci sono file associati a questo prodotto.
Pubblicazioni consigliate

Caricamento pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/187760
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus ND
  • ???jsp.display-item.citation.isi??? ND
social impact