We have studied the substrate specificities of a non-specific activator protein on the enzymatic hydrolyses of the following compounds: G(M1) and G(M2), as well as several of their derivatives including oligosaccharides, GgOse3Cer-II3-sulfate and LacCer-II3-sulfate, Gb-Ose3Cer and GgOse4Cer, three neolacto-series glycosphingolipids, and two non-ceramide glycolipids. Our results show that this activator protein has a broad spectrum of activity and exhibits the properties of a nonspecific natural detergent. The evidence of non-specificity was the ability of this activator protein to stimulate the hydrolyses of glycolipids, regardless of glycosphingolipids or non-ceramide glycolipids, carried out by glycosidases from animals, plants, and microorganisms. Its activity was, however, limited to substrates that had a lipid moiety. The oligosaccharide of G(M1) and deacetyl-fatty acid free G(M1) (II3-NeuGg-Ose4-sphingosine) were hydrolyzed by β-galactosidase in the absence of this activator protein.
CHARACTERIZATION OF A NONSPECIFIC ACTIVATOR PROTEIN FOR THE ENZYMATIC-HYDROLYSIS OF GLYCOLIPIDS / S. LI, S. SONNINO, G. TETTAMANTI, Y. LI. - In: THE JOURNAL OF BIOLOGICAL CHEMISTRY. - ISSN 0021-9258. - 263:14(1988), pp. 6588-6591.
|Titolo:||CHARACTERIZATION OF A NONSPECIFIC ACTIVATOR PROTEIN FOR THE ENZYMATIC-HYDROLYSIS OF GLYCOLIPIDS|
SONNINO, SANDRO (Secondo)
TETTAMANTI, GUIDO (Penultimo)
|Settore Scientifico Disciplinare:||Settore BIO/10 - Biochimica|
|Data di pubblicazione:||1988|
|Appare nelle tipologie:||01 - Articolo su periodico|