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EnglishMonoclonal antibody MOv2, produced against ovarian carcinoma, was previously found to bind a carbohydrate epitope (CAMOv2) present on mucins, glycoproteins and a neutral glycolipid. In this paper, the structure of the carbohydrate epitope is determined by immunological reactivity with purified glycolipids and oligosaccharides. Using solid-phase radioimmunoassay and immunostaining of thin layer chromatograms, MOv2 binds strongly to Le(a)-active pentasaccharide ceramide. A smaller neutral glycolipid also weakly binds MOv2. Fifty percent inhibition of binding to Le(a)-active pentasaccharide ceramide is achieved with approximately 8 μM concentration of lacto-N-fucopentaose II (LNF II). Lacto-N-tetraose (LNT) also partially inhibits at about 103 times higher concentration suggesting that the faster migrating glycolipid antigen contains this carbohydrate sequence. Binding to Le(a)-active hapten is further confirmed by the specific inhibition of binding by authentic anti-Le(a) monoclonal antibodies but not by anti-Le(b) monoclonal antibodies. The correlation between Le(a) phenotype and reactivity of MOv2 antibody in a serum assay among healthy blood donors also supports these results. In conclusion, we have obtained direct evidence from several independent experiments that antibody MOv2 recognizes the Le(a)-active hapten.
THE ANTITUMOR MONOCLONAL-ANTIBODY MOV2 RECOGNIZES THE LEWIS A-HAPTEN / F. LEONI, J. MAGNANI, S. MIOTTI, S. CANEVARI, M. PASQUALI, S. SONNINO, M. COLNAGHI. - In: HYBRIDOMA. - ISSN 0272-457X. - 7:2(1988), pp. 129-139.
| Titolo: | THE ANTITUMOR MONOCLONAL-ANTIBODY MOV2 RECOGNIZES THE LEWIS A-HAPTEN |
| Autori: | SONNINO, SANDRO (Penultimo) |
| Settore Scientifico Disciplinare: | Settore BIO/10 - Biochimica |
| Data di pubblicazione: | 1988 |
| Rivista: | |
| Tipologia: | Article (author) |
| Digital Object Identifier (DOI): | http://dx.doi.org/10.1089/hyb.1988.7.129 |
| Appare nelle tipologie: | 01 - Articolo su periodico |
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