The antiproliferative effect of a synthetic octapeptide, somatostatin analogue SMS 201-995 (SMS), and its capacity to bind were evaluated on human peripheral blood lymphocytes (PBL) activated by phytohemoagglutinin (PHA). We then addressed our work to investigate if SMS inhibits PHA activation of PBL by a cytostatic rather than a cytotoxic mechanism. Consequently, we studied the cell cycle distribution and the activation of caspase-3, measuring the presence of the cleavage product of poly(ADP-ribose) polymerases (PARP), and we evaluated the presence of apoptotic DNA by using a monoclonal antibody specific for the single-stranded regions of DNA. All our results indicate that SMS induces apoptosis in activated lymphocytes.

The apoptotic effect of somatostatin analogue SMS 201-995 on human lymphocytes / D. Lattuada, C. Casnici, A. Venuto, O. Marelli. - In: JOURNAL OF NEUROIMMUNOLOGY. - ISSN 0165-5728. - 133:1-2(2002 Dec), pp. 211-216. [10.1016/S0165-5728(02)00364-8]

The apoptotic effect of somatostatin analogue SMS 201-995 on human lymphocytes

D. Lattuada
Primo
;
O. Marelli
Ultimo
2002-12

Abstract

The antiproliferative effect of a synthetic octapeptide, somatostatin analogue SMS 201-995 (SMS), and its capacity to bind were evaluated on human peripheral blood lymphocytes (PBL) activated by phytohemoagglutinin (PHA). We then addressed our work to investigate if SMS inhibits PHA activation of PBL by a cytostatic rather than a cytotoxic mechanism. Consequently, we studied the cell cycle distribution and the activation of caspase-3, measuring the presence of the cleavage product of poly(ADP-ribose) polymerases (PARP), and we evaluated the presence of apoptotic DNA by using a monoclonal antibody specific for the single-stranded regions of DNA. All our results indicate that SMS induces apoptosis in activated lymphocytes.
Anti-Inflammatory Agents; Apoptosis; Humans; Caspase 3; Octreotide; Lymphocytes; Caspases; Chemotaxis, Leukocyte; Immunosuppressive Agents; Somatostatin; Cells, Cultured; Adult; DNA; Proteins; Middle Aged; Cell Cycle
Settore BIO/14 - Farmacologia
29-ott-2002
Article (author)
File in questo prodotto:
File Dimensione Formato  
1-s2.0-S0165572802003648-main.pdf

accesso riservato

Tipologia: Publisher's version/PDF
Dimensione 252.94 kB
Formato Adobe PDF
252.94 kB Adobe PDF   Visualizza/Apri   Richiedi una copia
Pubblicazioni consigliate

Caricamento pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/186710
Citazioni
  • ???jsp.display-item.citation.pmc??? 5
  • Scopus 35
  • ???jsp.display-item.citation.isi??? 28
social impact